摘要
为了探究提取长茎葡萄蕨藻多糖的最优工艺及抗氧化活性,对目前已有的多糖提取方法进行筛选,并采用单因素实验和响应面实验的方法,对料液比、提取温度、提取时间、木瓜蛋白酶添加量和提取次数这5个因素进行优化。结果显示,添加木瓜蛋白酶提取长茎葡萄蕨藻多糖的方法最高效便捷,且当料液比1∶40,提取温度50℃,提取时间3 h,提取次数2次,以及木瓜蛋白酶添加量为2.0%时,长茎葡萄蕨藻多糖提取率相对较高,可达到41.24%±0.09%。进一步的实验结果显示,长茎葡萄蕨藻多糖对DPPH和ABTS自由基均具有良好的清除活性,其IC50值分别为2.32和0.67 mg/mL。研究表明,使用优化后的木瓜蛋白酶酶解法能有效提高长茎葡萄蕨藻多糖的提取率,且长茎葡萄蕨藻多糖具有良好的抗氧化活性。本研究可为长茎葡萄蕨藻多糖的开发利用提供理论基础和参考依据。
Caulerpa lentillifera is a kind of perennial large edible seaweed,which is also known as"sea grape"because of its upright stem globule glittering and translucent,similar in appearance to grapes.C.lentillifera is also one of the most suitable seaweeds for human consumption.It is rich in nutrients and contains a variety of amino acids,vitamins,polysaccharides and fatty acids needed by the human body.Among them,polysaccharide is the most important active component in C.lentillifera.According to research,the crude polysaccharide content of C.lentillifera accounts for 34.00%-64.97%of its dry weight,which is higher than that of most edible algae,such as Scagassum,Hizikia fusifarme and Undaria pinnatifida.At present,the main extraction methods for polysaccharides of C.lentillifera are water extraction,alkali extraction and ultrasonic-assisted extraction,and the extraction rate is 3.22%-11.30%,but there are still a large amount of polysaccharides that remains un-extracted.In order to develop a mild,efficient and low-cost extraction process of polysaccharides from C.lentillifera,and to explore the antioxidant activity of the polysaccharide,we screened five existing extraction methods of polysaccharides from C.lentillifera.Through single factor experiment and response surface experiment,the ratio of material to solution,extraction temperature,extraction time,amount of papain and extraction times were optimized,and the optimum process parameters were obtained.Polysaccharide concentration was determined by anthrone sulfate method in the experiment.In addition,we investigated the scavenging activity of the polysaccharide on DPPH and ABTS free radicals.The results showed that adding papain is the most efficient method to extract polysaccharides from C.lentillifera.The order of the factors affecting the extraction rate of polysaccharide from C.lentillifera is as follows:ratio of material to solution>extraction temperature>extraction time,and the ratio of material to solution showed an extremely significant influence on the extr
作者
童艳梅
陈秀荔
胡庭俊
刘青云
李强勇
冯鹏霏
杨春玲
彭敏
朱威霖
潘传燕
曾地刚
赵永贞
TONG Yanmei;CHEN Xiuli;HU Tingjun;LIU Qingyun;LI Qiangyong;FENG Pengfei;YANG Chunling;PENG Min;ZHU Weilin;PAN Chuanyan;ZENG Digang;ZHAO Yongzhen(College of Animal Science and Technology,Guangxi University,Nanning 530005,China;Guangxi Academy of Fishery Science,Nanning 530021,China;Guangxi Shrimp Breeding Engineering Technology Research Center,Nanning 530021,China)
出处
《水产学报》
CSCD
北大核心
2024年第1期164-174,共11页
Journal of Fisheries of China
基金
广西科技计划项目“南美白对虾与长茎葡萄蕨藻工厂化生态共养模式研究”(桂科AB18221113)
国家虾产业技术体系建设专项(CARS-48)。
关键词
长茎葡萄蕨藻
多糖
木瓜蛋白酶
响应面法
抗氧化性
Caulerpa lentillifera
polysaccharide
papain
response surface method
antioxidant resistance
