摘要
目的研究医用胶原蛋白调节TNF-α治疗变应性接触性皮炎的作用及机制。方法制造二硝基氟苯致小鼠变应性接触性皮炎模型,医用胶原蛋白连续14 d涂抹动物皮肤,对耳部组织进行病理学检查,应用ELISA方法测试TNF-α、IL-2等炎症介质。结果与攻击前比较,各组小鼠在攻击后12、24 h均出现十分明显的耳变态反应(P<0.01);与空白对照组比较,医用胶原蛋白低剂量组小鼠在攻击后12 h时耳变态反应评分升高(P<0.05),在攻击后24 h时模型对照组耳变态反应评分明显升高(P<0.05);与模型对照组比较,在攻击后24 h时医用胶原蛋白低、中和高剂量组小鼠的耳变态反应评分均降低(P<0.05)。与空白对照组比较,模型对照组、地塞米松对照组和医用胶原蛋白低、中、高剂量组小鼠右耳片的炎症细胞数明显地增加(P<0.01);与模型对照组比较,地塞米松对照组和医用胶原蛋白低、高剂量组小鼠右耳片的炎症细胞数减少(P<0.05或P<0.01)。与空白对照组比较,模型对照组、地塞米松对照组和医用胶原蛋白中、高剂量组小鼠左右耳片厚度差明显地变大(P<0.01)。与模型对照组比较,医用胶原蛋白低剂量组小鼠左右耳片厚度差变小(P<0.05);与空白对照组比较,模型对照组和医用胶原蛋白低剂量组小鼠血清中炎症因子TNF-α含量明显地升高(P<0.01);与模型对照组比较,地塞米松对照组小鼠血清中炎症因子TNF-α含量明显地降低(P<0.01),医用胶原蛋白高剂量组小鼠血清中炎症因子TNF-α含量明显降低(P<0.05);对动物体重没有明显影响。结论医用胶原蛋白对变应性接触性皮炎有明显的治疗作用,可能通过调节血清中TNF-α进而减轻耳部炎性反应有关。
Objective To study the adjust TNF-α of medical collagen treats ACD and mechanism. Methods Duplication ACD models were made, the mouse skin was smeared with this medical collagen for about 14 days as a therapy, ear tissue were given histopathological examination, ELISA was used to test the levels of TNF-α,IL-2 inflammatory mediators. Results Compared with pre attack, ear allergy were very obvious in all group after attack 12, 24 h（P〈0.01）.Compared with blank control group, the ear allergy increased in medical collagen low does group after attack 12 h（P〈0.05）, the ear allergy increased in model control group after attack 24 h（P〈0.05）. Compared with model control group,the ear allergy reduced in medical collagen low, middle, high does group after attack 24h（P〈0.05）. Compared with blank control group, inflammatory cells of the right ear increased in all group mouse（P〈0.05）. Compared with model control group, inflammatory cells of the right ear reduced in dexamethasone control group, medical collagen low, high does group（P〈0.05 or P〈0.01）. Compared with blank control group, the left and right ear piece thickness difference enlarged obviously in model control group, dexamethasone control group, medical collagen middle and high does group（P〈0.01）. Compared with model control group, the left and right ear piece thick ness difference decreased in medical collagen low does group mouse（P〈0.05）. Compared with blank control group, TNF-α level increased obviously in model control group and medical collagen low does group mouse（P〈0.05）. Compared with model control group, TNF-α＇s level reduced in dexamethasone control group and medical collagen high does group mouse（P〈0.01 or P〈0.05）. Meanwhile, this therapy did not influenced the animals＇ weight. Conclusion Medical collagen can be a certain medical treatment for ACD, but it may connect with the adjust of the level of TNF-α and then decrease of the ear inflammation.
出处
《中国医药导报》
CAS
2017年第18期188-192,共5页
China Medical Herald
