摘要
目的:研究咪唑斯汀、氯雷他定及西替利嗪对变应性接触性皮炎(allergiccontactdermatitis,ACD)小鼠产生干扰素(IFN)、肿瘤坏死因子(TNF)及白介素(IL)-4的影响,探讨抗组胺药治疗ACD的作用机制。方法:建立小鼠ACD模型,采用ELISA法检测ACD小鼠产生IFN-γ、TNF-α和IL-4的水平及3种药物分别对它们的作用。结果:ACD小鼠血清及脾淋巴细胞IFN-γ水平均明显升高(P<0.01);血清TNF-α水平明显升高(P<0.01),腹腔巨噬细胞产生TNF-α水平无明显变化(P>0.05);血清及脾淋巴细胞产生IL-4水平无明显变化(P>0.05)。咪唑斯汀对血清及细胞培养液中3种细胞因子的水平均有显著或不同程度的抑制作用,而氯雷他定、西替利嗪仅对部分细胞因子产生一定的抑制作用。结论:咪唑斯汀对ACD小鼠产生的3种细胞因子的抑制作用强于氯雷他定和西替利嗪。它们对ACD小鼠3种细胞因子的抑制作用与临床疗效的关系有待进一步研究。
Objective： To investigate the effects of mizolastine on cytokine expression in murine allergic contact dermatitis（ACD） compared with loratadine and cetirizine, and to study the probable mechanisms of antihistamins to treat ACD. Methods： The animal model of allergic contact dermatitis was established, and the levels of IFN-γ TNF-α and IL-4 were detected by enzyme-linked immunosorbent assay （ELISA） in ACD mice treated with mizolastine, loratadine and cetirizine. Results： The levels of IFN-γin serum and the suspensions of spleen cell-culture as well as the levels of TNF-α in serum of ACD mice were significantly increased （P〈 0.01 ）, meanwhile the levels of TNF-α from peritoneal macrophages and IL-4 in serum and the suspensions of spleen cell-culture were not changed （P〉 0.05）. The levels of the three cytokines in serum and suspension of cell-culture were all depressed by mizolastine significantly or in different degrees, and the inhibitory effects of loratadine or cetirizine on the cytokines were partial and weaker. Conclusion： The inhibitory effects of mizolastine on the three cytokines of ACD mice are stronger than those of loratadine or cetirizine.
出处
《临床皮肤科杂志》
CAS
CSCD
北大核心
2006年第1期6-8,共3页
Journal of Clinical Dermatology
