摘要
目的观察续断对血管性痴呆(vascular dementia,VD)模型大鼠血流动力学的作用。方法 2014年3月—2015年10月选择48只大鼠随机分为假手术组、模型组、续断组和银杏叶组各12只。采用自制高脂饮食与改进的双侧颈总动脉永久结扎(2-VO)法制备高脂血症VD模型。假手术组和模型组给予生理盐水灌胃,续断组给予续断水煎剂8 g/(kg·d)灌胃,银杏叶组给予银杏叶提取物48 mg/(kg·d)灌胃,持续4周。用药结束,Y迷宫检测大鼠的认知功能,采血测血脂成分和血液黏度的改变情况。计量资料采用方差分析,两两比较采用LSD-t检验,P<0.05为差异有统计学意义。结果四组大鼠电击总数、出错总数及24 h后记忆出错总数比较差异均有统计学意义(均P<0.05)。模型组大鼠电击总数、出错总数及24 h后记忆出错总数较假手术组均显著增多,差异均有统计学意义(均P<0.05)。续断组与银杏叶组干预治疗后,大鼠学习记忆各指标较模型组均明显改善,差异均有统计学意义(均P<0.05)。四组TG、TC、LDL-C、Apo B100含量、全血黏度、血浆黏度、HDL-C和Apo AⅠ含量比较差异均有统计学意义(均P<0.05)。续断组与银杏叶组干预治疗后,大鼠TG、TC、LDL-C、Apo B100含量、全血黏度、血浆黏度均较模型组降低,HDL-C和Apo AⅠ含量均较模型组升高,差异均有统计学意义(均P<0.05)。结论续断通过降低VD大鼠的血脂水平、纠正脂类代谢紊乱和降低血液黏度等机制,改善VD模型大鼠的学习记忆能力,对VD大鼠具有较好的防治作用。
Objective To investigate the effects of dipsacus asperoides on blood dynamics in vascular dementia（VD） model rats. Methods A total of 48 rats were randomly divided into sham operation group, model group, dipsacus asperoides group and ginkgo biloba group（n=12） from March 2014 to October 2015. Using homemade high fat feed and improved permanent bilateral common carotid artery ligation（2-VO） preparation of hyperlipidemia VD rat model. Rats in sham operation group and model group were orally administered with volume distilled water, and rats in dipsacus asperoides group with water extract of dpsacus asperoides, rats in ginkgo biloba group with extract of ginkgo biloba, once daily for 4 weeks.After the treatment, the cognitive function was examined by using Y maze test, blood lipid and blood viscosity measurement followed. One-way ANOVA was used for measurement date, further analysis within groups with LSD-t test, P〈0.05 was considered statistically significant. Results The total number of electric shocks, the errors and the errors of memory test after 24 h were statistically significant among the four groups（all P〈0.05）. The total number of electric shocks, the errors and the errors of memory test after 24 h significantly increased in model rats compared with the sham group, the differences were statistically significant（all P〈0.05）. While the above indicators were significantly decreased in dipsacus asperoides group and ginkgo biloba group than that in the model group, the differences were statistically significant（all P〈0.05）. The content of TG,TC,LDL-C,Apo B100,whole blood viscosity,plasma viscosity,HDLC,Apo AⅠwere statistically significant among the four groups（all P〈0.05）. The content of TG,TC,LDL-C,Apo B100,whole blood viscosity,plasma viscosity were reduced, and the content of HDL-C and Apo A Ⅰ were increased in dipsacus asperoides group and ginkgo biloba group compared with the model group, the differences were statistically significant（all P〈0.05）. ConclusionDi
出处
《社区医学杂志》
2016年第14期1-3,共3页
Journal Of Community Medicine
基金
南阳市科技攻关项目(2012GG050)
关键词
续断
血管性痴呆
学习记忆
血脂
血液黏度
Dipsacus asperoides
Vascular dementia
Blood lipid
Blood viscosity
