摘要
目的:建立续断药材HPLC指纹图谱分析方法。方法:采用HPLC法测定了全国不同产地20批续断样品。色谱条件:色谱柱W elchrom-C18(250 mm×4.6 mm,5μm),乙腈-水为流动相进行梯度洗脱,流速1.0 mL/m in,检测波长212 nm,柱温35℃。结果:建立了续断皂苷类成分的HPLC指纹图谱共有模式,并对全国不同产地续断药材进行了相似度比较。结论:该分析方法稳定可靠,信息量大,为续断药材的鉴别和质量评价与控制提供了依据。
Objective：To establish the HPLC fingerprints of Dipsacus asperoides for reflecting the internal information,evaluating its internal quality.Methods：20 batches of D.asperoides were collected from different place with the HPLC fingerprints method.Chromatographic column：Welchrom-C18（250 mm×4.6 mm,5 μm）,mobile phase：acetonitrile and water（gradient elution）,flow rate：1.0 mL/min,detection wavelength：212 nm,column temperature：35 ℃.Results：The common mode of HPLC fingerprint was established and similar degrees to D.asperoides from different areas were compared.Conclusion：The method is stable,reliable,and with full information which can be used for quality evaluation,quality control item and crude drug indentification of D.asperoides.
出处
《中药材》
CAS
CSCD
北大核心
2010年第3期359-361,共3页
Journal of Chinese Medicinal Materials
基金
国家环境保护总局项目(YSZ-428-07)
关键词
续断
HPLC
指纹图谱
Dipsacus asperoides
HPLC
Fingerprint
