摘要
采用薄层层析法(TLC)对四味健蚕增茧液中的白头翁药材进行鉴别,以硅胶G-CMCNa薄层板为固定相,氯仿-甲醇-水(70:30:10)作为展开剂,展开后喷10%H2SO4,105℃加热5～10 min显色,能较好地分离四味健蚕增茧酊中的各种成分,从而对处方中的白头翁进行质量监控。本方法简便、准确,重现性好,专属性强,可对复方中的白头翁药材进行定性和半定量检查。
Thin Layer Chromatography （TLC） was employed to detect the content of＂Radix Pulsatillae＂ in Si wei jian can zeng jian ye, with silicon gel G-CMCNa plates as the stationary phase, chloroform/methanol/water （70： 30：10） as the solvent, spraying 10% H2SO4 after separation and heated at 105 degree for 5-10 minutes to visualize the spots. This method could effectively monitor the ＂Radix Pulsatillae＂ content in Si weijian can zengjian ye. It was simple, specific, repeatable and suitable for qualifying and semiquantifying ＂Radix Pulsatillae＂ in mixture.
出处
《广东畜牧兽医科技》
2010年第1期35-36,共2页
Guangdong Journal of Animal and Veterinary Science
基金
广东省科技计划项目(2007B030801002)
关键词
薄层层析法
白头翁
四味健蚕增茧液
Thin Layer Chromatography （TLC）
radix pulsatillae
Si weijian can zengjian ye
