摘要
目的探讨续断对血管性痴呆模型大鼠学习记忆障碍的改善作用及其机制。方法将48只Wistar大鼠随机分为假手术组、模型组、续断低剂量组和续断高剂量组,每组12只。除假手术组外,其余组均采用双侧颈总动脉永久结扎法制备血管性痴呆模型。大鼠清醒后,续断低、高剂量组分别给予续断水煎剂2 g/(kg·d)和8 g/(kg·d)灌胃,假手术组和模型组给予等容量0.9%Na Cl溶液灌胃,均1次/d,持续4周。灌胃结束后,采用Morris水迷宫测定各组大鼠的学习记忆功能;黄嘌呤氧化酶法测定各组大鼠脑组织中超氧化物歧化酶(SOD)活性,硫代巴比妥酸法测定丙二醛(MDA)含量。结果与假手术组相比,模型组大鼠游泳总距离明显延长(P<0.05),目标象限游泳总距离明显缩短(P<0.05),穿过站台总数明显减少(P<0.05);大鼠前额叶皮质MDA含量明显升高(P<0.05),SOD活性明显降低(P<0.05)。与模型组比较,续断低、高剂量组大鼠游泳总距离明显缩短(P均<0.05),目标象限游泳总距离明显延长(P均<0.05),穿过站台总数明显增多(P均<0.05);大鼠前额叶皮质MDA含量明显降低(P均<0.05),SOD活性明显升高(P均<0.05)。与续断低剂量组比较,续断高剂量组各指标改善情况更明显(P均<0.05)。结论续断可能是通过对抗或减少血管性痴呆大鼠体内氧自由基来发挥其对认知功能障碍的改善作用。
Objective It is to explore the improving effect and its mechanism of Himalayan Teasel Root （ HTR） on the learning and memory dysfunction of rat models with vascular dementia .Methods 48 Wistar rats were randomly divided into sham operation group, model group, low and high dose of HTR groups, each group had 12 rats.Bilateral common carotid ar-teries of all the rats except in sham operation group were ligated to establish vascular dementia models .After the rats awake , the ones in low and high dose of HTR groups were given HTR decoction 2 g/（ kg· d） and 8 g/（ kg· d） by lavage respective-ly, the ones in sham operation group and model group were given the same dose of normal saline by lavage , all the rats were treated once per day and for 4 weeks.At the end of treatment , learning and memory function of the rats were tested by Morris water maze test , the activity of SOD and content of MDA in cerebral tissue were determined by Xanthine oxidase NBT method and Thiobarbituric acid method .Results Compared with sham operation group , the rats＇total swimming distance was longer （ P〈0.05）, total swimming distance in target quadrant was shoter （P〈0.05）, times of passing platform were fewer （P〈0. 05）, the content of MDA in prefrontal cortex was higher while the activity of SOD was lower in model group （P〈0.05）. Compared with model group, the rats＇total swimming distance was shorter （P〈0.05）, total swimming distance in target quad-rant was longer （P〈0.05）, times of passing platform were more （P〈0.05）, the content of MDA in prefrontal cortex was lower while the activity of SOD was higher in low and high dose of HTR groups （P〈0.05）.Compared with low dose of HTR group, the improvements of the indexes above were more significant （P〈0.05）.Conclusion HTR can improve cognitive function of the rats with vascular dementia might by inhibit or decrease the oxygen free radicals in the rats .
出处
《现代中西医结合杂志》
CAS
2016年第22期2408-2410,共3页
Modern Journal of Integrated Traditional Chinese and Western Medicine
基金
南阳市科技攻关项目(2012GG050)
