摘要
目的:观察小青龙汤对肺源性心脏病大鼠血浆血管紧张素Ⅱ(AngⅡ)、醛固酮(ALD)、钠钾泵(Na^+-K^+-ATP酶)的含量和血浆中AT_1和AT_2mRNA表达的影响,以进一步探讨宣肺利水法的可能作用机理。方法:Wistar大鼠60只,每组20只,随机分为正常组、模型组和小青龙汤组;利用酶联免疫吸附测定法(ELISA)测定AngⅡ、ALD及Na^+-K^+-ATP酶的含量,并用荧光定量PCR检测AT_1和AT_2mRNA的表达。结果:与正常组比较,模型组的AngⅡ、ALD及Na^+-K^+-ATP酶含量明显升高(P<0.05或P<0.01);与模型组比较,小青龙汤组的AngⅡ、ALD及Na^+-K^+-ATP酶含量明显降低(P<0.05)。与正常组比较,模型组的AT_1 mRNA表达有所上升,AT_2mRNA表达有所降低(P<0.05或P<0.01);与模型组比较,小青龙汤组的AT_1 mRNA表达有所下降,AT_2mRNA表达有所升高(P<0.01)。结论:小青龙汤可有效调节血浆中AT_1和AT_2mRNA的表达,影响ALD的含量,从而发挥宣肺利水的作用。
This article was aimed to study the effect of Xiao-Qing-Long Decoction（XQLD） on plasma Ang Ⅱ, ALD, Na^＋-k^＋-ATPase content, and plasma AT1 and AT2mRNA expressions in Cor pulmonale rats, in order to further explore the mechanism of ventilating lung qi for diuresis. A total of 60 Wistar rats were randomly divided into the normal group, model group and XQLD, with 20 rats in each group. The enzyme-linked immunosorbent assay（ELISA） method was used to determine the AngⅡ, ALD and Na^＋-k^＋-ATPase content. The fluorescence quantitative PCR was used to detect the AT1 and AT2mRNA expression. The results showed that compared with the normal group, the AngⅡ, ALD and Na^＋-k^＋-ATPase contents in the model group were significantly increased（P〈0.05, or P〈0.01）. Compared with the model group, the AngⅡ, ALD and Na^＋-k^＋-ATPase contents in the XQLD group were obviously decreased（P〈0.05）. Compared with the normal group, AT1 mRNA expression was increased; and AT2mRNA expression was decreased in the model group（P〈0.05, or P〈0.01）. Compared with the model group, AT1 mRNA expression was decreased; and AT2mRNA expression was increased in the XQLD group（P〈0.01）. It was concluded that XQLD can effectively regulate the AT1 and AT2mRNA expressions, influence ALD content to ventilate lung qi for dieresis.
出处
《世界科学技术-中医药现代化》
2015年第10期2104-2108,共5页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
国家自然科学基金委青年基金项目(81001520):应用宣肺利水治法探讨"肺主行水"机制的实验研究
负责人:彭波
关键词
小青龙汤
宣肺利水法
血管紧张素Ⅱ
荧光定量
Xiao-Qing-Long Decoction
ventilating lung qi for dieresis
AngⅡ
fluorescence quantitative
