摘要
目的:观察肺卫失宣大鼠肺组织Na+-K+-ATP酶活力及肺泡Ⅱ型上皮细胞超微结构的变化及大黄的调节作用,探讨肺卫失宣肺损伤的物质基础及大黄的保护机制。方法:以内毒素法建立肺卫失宣大鼠模型。60只大鼠随机分成:正常对照组、肺卫失宣模型组、模型自然恢复5 d组、大黄预防组(大黄颗粒剂9 g·kg-1.d-1,ig连续2 d后造模)和大黄治疗组(造模后ig大黄颗粒剂9 g·kg-1.d-1,连续5 d)。采用考马斯亮蓝蛋白测定试剂盒检测Na+-K+-ATP酶活力并以透射电镜观察肺泡Ⅱ型上皮细胞超微结构的变化。结果:与正常肺泡Ⅱ型上皮细胞超微结构相比,肺卫失宣模型组肺泡Ⅱ型上皮细胞出现了板层小体空泡化,内质网扩张,胞浆肿胀,核周隙扩张等;自然恢复5 d组则表现为板层小体大量空泡化,核固缩显著等超微结构改变;大黄干预后,板层小体数目增多,内质网无扩张。与正常对照组比较,肺卫失宣模型组Na+-K+-ATP酶活力呈降低趋势,而自然恢复5 d组酶活力水平却异常升高(P<0.05);大黄预防或治疗给药对Na+-K+-ATP酶高活性或低活力呈抑制作用。结论:肺卫失宣大鼠肺泡Ⅱ型上皮细胞超微结构受到破坏,Na+-K+-ATP酶活力异常,提示肺卫失宣大鼠肺内微环境发生改变。大黄对肺泡Ⅱ型上皮细胞的超微结构损伤具有修复作用,并对Na+-K+-ATP酶活力调节呈双向调节作用,是其保护肺卫失宣肺损伤的重要作用机制。
Objective：To observe the changes of Na ＋-K ＋-ATPase activity in the lung tissue and the changes of ultra-structure in alveolar type Ⅱ cells in rats with lung defensive sluggishness syndrome（LDSS） and the regulation of rhubarb on it to explore the material foundation of LDSS and the mechanism of rhubarb in the prevention and treatment of the lung injury.Method：Lipopolysaccharide（LPS）was injected into SD rats to perform lung-defensive sluggishness syndrom,60 rats was randomizly divided into 5 groups：control group,lung-defensive sluggishness group,natural recovery after 5 days group,Rhubarb prevention group（9 g.kg-1.d-1 for 2 days beforemodle establishment） and rhubarb treatment group（9 g.kg-1.d-1 for 5 days after model establichment）.The ultrastructure changes were observed under transmission electron microscope,The homogenate was used to detect the activity of Na ＋-K ＋-ATPase with the Na ＋-K ＋-ATPase kit.Result：Compared with the normal control group,alveolar type Ⅱ cells Lamellar body vacuoles,endoplasmic reticulum,cytoplasmic swelling and perinuclear extension were showed in the model group.A large number of alveolar type Ⅱ cells Lamellar body vacuoles,endoplasmic reticulum,markedly nuclear condensation were showed in the natural recovery after 5 days group.After the rhubarb intervention,the number of lamellar body of type Ⅱ cells was larger than that in recovery after 5 days group and lung-defensive sluggishness group,especially no enlargement of endoplasmic reticulum.It was showed that the expression of Na ＋-K ＋-ATPase activity of lung-defensive sluggishness group was lower than that of control group.However,the expression in the natural recovery after 5 days group was significant higher than control group.Rhubarb could inhibit the abnormal expression of Na ＋-K ＋-ATPase activity of lung.Conclusion：The abnormal ultra-structure of alveolar type Ⅱ cells and abnormal expression of Na ＋-K ＋-ATPase activity can be showed in lung-defensive sluggishness model
出处
《中国实验方剂学杂志》
CAS
北大核心
2011年第8期190-193,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
广东省科技厅项目(83028
2009B060300025)
广东省中医药管理局项目(2008318)
广州中医药大学项目(08CX82)
