摘要
目的:研究泻肺化痰祛瘀法对野百合碱诱发肺动脉高压大鼠的疗效和机制。方法:利用野百合碱诱发大鼠肺动脉高压模型。清洁级健康雄性W istar大鼠60只,随机分为正常对照组、模型组、泻肺化痰祛瘀法中药低剂量组和中药高剂量组。于制模15 d起,两个中药组每日晨起以中药制剂(由制大黄9 g,炙甘遂2 g,葶苈子15 g,川芎9 g,黄芩15 g组成)灌胃1次,连续7 d;正常对照组和模型组以同法灌胃生理盐水。利用右心导管插管术测定右心室平均压(M RVP)和肺动脉平均压(M PAP);以苏木素伊红(HE)染色法在光镜下进行肺组织病理检查。结果:中药高剂量组大鼠M RVP〔(13.37±4.22)mm Hg,1 mm Hg=0.133 kPa〕和M PAP〔(12.26±2.79)mm Hg〕均较模型组〔分别为(16.27±2.27)mm Hg和(14.77±2.39)mm Hg〕降低(P<0.05或P<0.01),而中药低剂量组改善M RVP和M PAP的作用与高剂量组相当,但与模型组比较差异无显著性。中药高剂量组右心室及肺组织病理改变也较中药低剂量组和模型组减轻。结论:泻肺化痰祛瘀法具有降低肺动脉压、防止肺血管重构的作用。
Objective： To study the efficacy of purging the lung and removing phlegm and blood stasis method （泻肺化痰祛瘀法） on animal model of pulmonary hypertension induced by monocrotaline and explore its mechanism. Methods： The model of pulmonary hypertension induced by monocrotaline was established in rats. Sixty healthy and clean male Wistar rats were randomly divided into normal control group, model group, low and high dosage of traditional Chinese medicine （TCM） drugs for purging the lung and removing phlegm and blood stasis groups. From the 15 th day after model establishment, two dosage groups were treated with the drugs in the prescription containing the main ingredients as follows： 9 g prepared rhubarb （制大黄）, 2g prepared euphorbia kansui （炙甘遂）, 15 g lepidium apetalum （葶苈子）, 9 g ligusticum （川芎） and 15 g scutellaria （黄芩） infused into the stomach once a day for 7 days. Normal control group and model group were given normal saline infused into the stomach. Mean right ventricular pressure （MRVP） and the mean pulmonary artery pressure （MPAP） of rats were measured by right cardiac catheterization, and the pathological changes of lung tissues were detected by hematoxylin and eosin （HE） stained sections. Results： The MRVP and the MPAP in high dosage group [（13.37±4.22）mm Hg and （12.26±2.79） mm Hg, 1 mm Hg=0. 133 kPa] were lower than that in the model group [（16. 27±2.27） mm Hg and （14.77±2.39） mm Hg, P〈0.05 or P〈0.01]. The effect of low dosage of TCM drugs on improving MRVP and MPAP was similar to that of the high dosage of TCM drugs, but the difference was not significant between the low dosage group and the model group. The pathological changes of right ventricular and lung tissues were better in high dosage group compared with those of the low dosage group and model group. Conclusion： TCM drugs for purging the lung and removing phlegm and blood stasis could decrease pulmonary artery pressure and might prevent pul
出处
《中国中西医结合急救杂志》
CAS
2006年第3期153-155,F0003,共4页
Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care
关键词
泻肺祛痰祛瘀法
肺动脉高压
病理学
大鼠
purging the lung and removing phlegm and blood stasis method
pulmonary hypertension
pathology
