摘要
目的 探讨高压氧预处理（hyperbaric oxygen preconditioning,HBOP）对急性高海拔缺氧体力负荷人体血清白细胞介素-6（interleukin-6,IL-6）及基质金属蛋白酶-9（matrix metalloproteinase-9,MMP-9）的影响.方法 8名成年男性自愿参加本研究,对受试对象在海平面、急性4000m高海拔暴露以及HBOP3、5和7d后急性4000m高海拔暴露条件下安静和体力负荷后的血清IL-6和MMP-9含量进行测定.结果 机体在急性高海拔暴露体力负荷后的血清IL-6和MMP-9[（29.56±1.26） ng/L、（285.25±11.67）mg/L]、均较海平面对照值[（27.74±1.06） ng/L、（269.88±9.90） mg/L]明显升高,差异有统计学意义（P＜0.01或P＜0.05）;HBOP 5 d后急性高海拔暴露机体安静时的血清IL-6和MMP-9水平[（23.26±1.25） ng/L、（230.28±11.96） ng/L]均较4000m对照值[（28.96±1.38） ng/L、（281.50±12.32） ng/L]明显降低,差异有统计学意义（均P＜0.01）;HBOP 3 d、7d后急性暴露于4000m体力负荷后的血清IL-6和MMP-9水平均较4 000 m对照值明显降低（均P＜0.01）,且HBOP 7 d后的IL-6和MMP-9水平均较HBOP3d组显著降低,差异有统计学意义（均P＜0.01）.结论 人体急性暴露于高海拔时血清IL-6和MMP-9明显增高,HBOP可显著降低急性高海拔暴露人体安静和体力负荷后的IL-6和MMP-9水平,能有效防止缺氧机体的功能障碍和组织损伤,从而提高机体在高海拔环境下的劳动能力,而且经HBOP7 d后的效果更为显著.
Objective To investigate the effects of hyperbaric oxygen preconditioning （HBOP） on the serum levels of interleukin-6 （IL-6） and matrix metalloproteinase-9 （MMP-9） during physical load at acute hypoxia,and possible mechanism involved.Methods Eight healthy male volunteers ere enrolled in the study.Serum levels of IL-6 and MMP-9 were detected by ELISA under the following conditions：at sea level and during acute exposure to 4 000 m high altitude ; as well as during acute exposure to 4 000 m high altitude both at rest and with physical load,following 3-day,5-day and 7-day HBOP respectively.Results Serum levels of IL-6 and MMP-9 at 4 000 m high altitude and after physical exercise were greatly increased [（29.56 ± 1.26） ng/L、（285.25 ± 11.67）] mg/L],as compared with the control value at the sea level （27.74 ± 1.06）] ng/L、（269.88 ± 9.90） mg/L] （P 〈 0.01 or P 〈 0.05）.After 5-day HBOP,serum levels of IL-6 and MMP-9 at rest and during acute hypoxia were greatly decreased [（23.26 ± 1.25） ng/L、（230.28 ± 11.96） ng/L],as compared with the control value at 4 000 m[（28.96 ± 1.38）ng/L、（281.50 ± 12.32） ng/L] （P 〈 0.01）.After 3-day and 7-day HBOP,serum levels of IL-6 and MMP-9 at acute hypoxia were greatly decreased after physical load,as compared with the control value at 4 000 m（P 〈0.01）.Furthermore,serum levels of IL-6 and MMP-9 after 7-day HBOP were significantly lower than those after 3-day HBOP（P 〈 0.01）.Conclusions Serum levels of IL-6 and MMP-9 could be significantly increased after acute exposure to high altitude.HBOP could not only obviously decrease serum levels of IL-6 and MMP-9 at acute hypoxia,both at rest and after physical exercise,but effectively prevent functional disorder and tissue lesion induced by acute hypoxia,thus promoting physical performance at acute hypoxia.Furthermore,7-day HBOP could produce more significant effects.
出处
《中华航海医学与高气压医学杂志》
CAS
CSCD
2015年第1期8-11,共4页
Chinese Journal of Nautical Medicine and Hyperbaric Medicine
