摘要
目的探讨高压氧（HBO）预处理对急性低氧大鼠心肌超微结构和心脏功能的影响。方法取13只Wistar大白鼠随机分为2组：低氧组（n=7），将大鼠置于模拟海拔5400m低压氧舱内3d；HBO预处理组（HBO组，n=6），进入低压舱前2d给予HBO预处理2d，每天1次，然后置于模拟海拔5400m低压氧舱内3d。第4天上午在模拟海拔5400m的低压氧舱内，2组动物用10％氨基甲酸乙酯腹腔麻醉，经右颈外静脉插入心导管至右心室、肺动脉，用多道生理记录仪连续描记肺动脉压力曲线5min，测定心率（HR）、肺动脉压力（PAP）、右室收缩压（RVSP）和舒张压（RVEDP）、右室等容收缩期心室内压力上升最大速率和右室等容舒张期心室内压力下降最大速率（-±dp／dtmax）等血流动力学指标，并观察心肌超微结构的变化。结果HBO组较低氧组HR、RVSP降低，±dp／dtmax增高，差异有统计学意义（P〈0．01）；肺动脉舒张压降低，差异有统计学意义（P〈0．05），肺动脉收缩压、肺动脉平均压、RVEDP差异无统计学意义（P〉0．05）。低氧组心肌细胞线粒体肿胀、溶解，肌浆网扩张，局部区域形成许多泡状；HBO组心肌无明显病变，仅滑面内质网轻度扩张。结论高压氧具有改善急性低氧大鼠血流动力学、降低肺动脉压、保护心功能的作用。
Objective To explore effects of hyperbaric oxygen （HBO） preconditioning on cardiac muscle uhramicrostructure and heart function in acute hypoxia rats. Methods Thirteen Wistar rats were randomly divided into two groups. The rats in hypoxia group（n = 7） were put into analogic low pressure oxygen of 5400 m altitude for 3 days and the rats in HBO preconditioning group（n = 6） were firstly treated by HBO preconditioning once per day for 2 days, then put into analogic low pressure oxygen of 5400 m altitude for 3 days. All rats were anesthetized by 10% urethane through abdominal cavity in the analogic low pressure oxygen of 5400 m altitude on the morning of the 4th day. Pulmonary arterial pressure plot were traced with polygraph for 5 min and haemodynamics indexes were detected such as heart rate （HR）, pulmonary arterial pressure （PAP）, right ventricular systolic pressure （RVSP）, right ventricular end-diastolic pressure （RVEDP） and maximum rate of rise/drop of right ventricular pressure （±dp/dtmax） etc and the changes of cardiac muscle ultrastructure were observed. Results Compared with hypoxia group, HR and RVSP decreased and ±dp/dtmax increased in HBO preconditioning group, there were significant differences between the two groups （P 〈 0. 01 or P 〈 0. 05）. There was no difference in pulmonary arterial systolic pressure, pulmonary artery mean pressure and RVEDP between the two groups （ P 〉 0.05 ）. In hypoxia group, cadiocyte bioblast was intumesced and dissolved, and sarcoplasmic reticulum was distended and hydatid formed at partial area. In HBO group, there was no obviously change in cadiocyte bioblast and sarcoplasmic reticulum was gently distended. Conclusions Hyperbaric oxygen can improve haemodynamics＇in acute hypoxia rats and degrade pulmonary arterial pressure and protect heart function.
出处
《中华航海医学与高气压医学杂志》
CAS
CSCD
2008年第5期276-278,共3页
Chinese Journal of Nautical Medicine and Hyperbaric Medicine
基金
全军“十一五”医药卫生科研基金资助项目（06MA091）
关键词
高原
高压氧
心脏功能
血流动力学
超微结构
High altitude
Hyperbaric oxygen
Heart function
Haemodynamics
Ultramicrostructure
