摘要
目的:探讨NAC基因结构和分布特点,了解NAC基因之间的进化关系。方法:从NCBI下载获得目标序列,通过染色体定位、比对、拼接得到新的mRNA,翻译为蛋白质,得到保守基序,再比对,绘制系统发育树进行分析。结果:生物信息学分析发现NAC家族基因在5个物种的基因组中都有分布;大多数NAC基因都有3个以上的外显子,绝大多数NAC基因仅1个NAM结构域,大多数NAM结构域都具有两端的保守基序,大多数NAM结构域位于2个相邻外显子上;NAC蛋白在单、双子叶植物分化上已有功能上的分歧。结论:NAC家族基因分布广,结构多为3个外显子,1个拥有两端保守基序的NAM结构域位于2个相邻外显子上。该研究为鉴定并获得NAC全长序列奠定了基础。
Objective： To understand the structure and distribution of NAC gene and to analyze the evolutionary relationships. Methods： The target sequences of NAC mRNA/cDNA sequences were downloaded from GenBank database and mapped to the chromosomes through the softwares of sequence alignment and chromosomal localization. The proteins translated from the new mRNAs of NAC family from genomic DNA sequences in chromosomes were aligned and used to the phylogenetic analysis. Results： Bioinformatics analysis revealed that NAC family genes were distributed in every genome of five species. Most NAC genes had three or more exons. The majority of the NAC genes had only one NAM domain. The end of most NAM domain had a conserved motif. Most of NAM domain located between two adjacent exons. The function difference of NAC proteins has been appeared before single and double monocots differentiation. Conclusion： NAC family genes are distributed in a wide range. Most of them have 3 exons, and the NAM domain are located between 2 adjacent exons. It＇s beneficial to understand the structure and functions and get their full-length sequence through this research.
出处
《生物技术通讯》
CAS
2015年第1期68-73,共6页
Letters in Biotechnology
基金
国家自然科学基金(31271302)
