摘要
【目的】研究柳树重要抗逆转录因子基因家族,为揭示柳树抗逆分子机制提供理论依据。【方法】以‘苏柳2345'的转录组测序数据为基础,克隆了柳树NAC家族,并分析其序列结构、组织表达特异性以及不同胁迫条件下的表达模式。【结果】克隆的两个柳树NAC转录因子基因分别命名为SlNAC1和SlNAC2。生物信息学分析表明:SlNAC1序列全长为1126 bp,编码产物含343个氨基酸残基,为稳定的亲水蛋白,定位于细胞核;SlNAC2序列全长为1139 bp,编码产物含291个氨基酸残基,为稳定的亲水蛋白,定位于叶绿体。两个基因均具有典型的NAM结构域及A、B、C、D、E 5个亚结构域,还包括共同的LPPG、YPNG和DEE保守基序及NAC抑制结构域。系统进化树分析显示,SlNAC1基因与木薯亲缘关系最近,SlNAC2基因与茄子亲缘关系最近。定量PCR结果显示SlNAC1和SlNAC2均在叶片表达,根中没有表达,为叶片特异性转录因子。定量PCR结果显示SlNAC1基因在脱落酸(ABA)和赤霉素(GA)处理24 h后显著上调,SlNAC2基因在聚乙二醇(PEG)、ABA和GA胁迫后显著上调。【结论】柳树SlNAC1基因在非生物胁迫下表达较为稳定,受ABA和GA胁迫诱导;SlNAC2受干旱、ABA和GA胁迫诱导,受影响明显高于SlNAC1,不受乙烯剂(ETH)胁迫影响。推测SlNAC1和SlNAC2参与GA、ABA信号传导,可能不参与ETH的信号途径。
【Objective】NAC transcription factors play an important role in regulating plant growth,development and response to stress tolerance.Studying the sequence,structure,evolutionary relationship and expression pattern of NAC transcription factor gene family related with stress tolerance in willow will have important theoretical significance for understanding the molecular mechanism underlying stress tolerance,which in turn will provide a theoretical basis for molecular assisted breeding in future.【Method】Two members of the NAC gene family were cloned based on the RNA-seq data of the Salix×jiangsuensis‘J2345'variety.The gene structure,protein properties and gene phylogeny were analyzed by using bioinformatics.The tissue specific expression in leaf and root,as well as the expression patterns under different types of stress was detected by quantitative real time PCR.【Result】The two NAC transcription factors cloned from the leaves of Salix×jiangsuensis‘J2345'variety were named SlNAC1 and SlNAC2.Bioinformatics analysis results showed that the sequence lengths of SlNAC1 and SlNAC2 were 1126 bp and 1139 bp,encoding proteins with 343 and 291 amino acid residues,respectively.The molecular weight of the proteins expressed from the two SlNAC1 and SlNAC2 genes were 40.1 ku and 42.3 ku,respectively,and both were stable,hydrophilic proteins.The SlNAC1 gene was located in nucleus while the SlNAC2 gene was located in chloroplast.Sequence alignment showed that both the genes contained typical NAM domains as well as A,B,C,D and E sub-domains,which shared the LPPG,YPNG and DEE conservative motifs and NAC suppression domains.Phylogenetic analysis demonstrated that SlNAC1 and SlNAC2 shared the highest homology with the genes from Manihot esculenta and Solanum melongena,respectively.RT-PCR results showed that both SlNAC1 and SlNAC2 were expressed in leaves and not in roots.The results of qRT-PCR showed that SlNAC1 was significantly upregulated after 24 hours of abscisic acid(ABA)and gibberellins(GA)treatments.SlNAC2 was s
作者
田雪瑶
周洁
王保松
何开跃
何旭东
TIAN Xueyao;ZHOU Jie;WANG Baosong;HE Kaiyue*;HE Xudong(College of Biology and the Environment,Nanjing Forestry University,Nanjing 210037,China;Jiangsu Academy of Forestry,Nanjing 211153,China;Willow Nursery of the Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm,Nanjing 211153,China)
出处
《南京林业大学学报（自然科学版）》
CAS
CSCD
北大核心
2020年第1期119-124,共6页
Journal of Nanjing Forestry University：Natural Sciences Edition
基金
国家自然科学基金项目(31300556)
江苏省林业科学研究院自主科研项目(BM2018022-01)
