摘要
利用NTT(核蛋白筛选系统)从大豆耐盐品种铁丰八号中克隆到1个新的NAC转录因子基因GmNAC2a,该基因DNA片段长度为900 bp,编码299个氨基酸,N端包含1个NAM保守域.系统进化树分析表明,GmNAC2a属于ATAF亚族,GmNAC2a与花生AhNAC1亲缘关系最近,同源性达到76.47%.GmNAC2a蛋白经亚细胞定位分析定位于细胞核中.基因表达分析发现,GmNAC2a基因对干旱、高温、低温、高盐、ABA、乙烯等多种胁迫均有响应,其中对乙烯响应最为敏感.研究表明GmNAC2a基因可能作为交叉点,不仅参与非生物胁迫响应途径,而且可能参与乙烯相关的生物响应途径.
In this study,a novel member of NAC transcription factor gene named GmNAC2a,was isolated from soybean（Glycine max L.var.＇Tiefeng 8＇,salt tolerance variety） using NTT（Nuclear Transportation Trap） system.The GmNAC2a contains 900 bp,encoding 299 amino acids with a conserved NAM domain in N terminal of protein.The phylogenetic analysis showed that GmNAC2a has the highest similarity with AhNAC1 from Arachis hypogaea（76.47%）,and the GmNAC2a belongs to the ATAF subfamily in NAC family.Subcellular localization analysis showed that GmNAC2a located in the nucleus.The Real-Time PCR indicated that expression of GmNAC2a were induced by drought,high temperature,low temperature,salt,ABA and ethylene stress,and it is the most sensitive to the ethylene.The results suggest that GmNAC2a might be the crosstalking point,which involved in both abiotic and ethylene related biotic stress response pathways.
出处
《西北植物学报》
CAS
CSCD
北大核心
2011年第1期8-13,共6页
Acta Botanica Boreali-Occidentalia Sinica
基金
国家863计划(2008AA10Z124和2006AA10A111)
国家自然科学基金(30700508)
关键词
大豆
NAC转录因子
基因克隆
表达分析
soybean
NAC transcription factors
gene isolation
expression analysis
