摘要
MicroRNAs(miRNAs)对植物抗逆及生长发育过程起着重要的调控作用,而miR164是植物特有的miRNA,它对应的靶基因主要是NAC转录因子家族。采用生物信息学方法对葡萄以及其他几个物种的miR164家族成员前体进行进化分析、前体二级结构预测、成熟序列碱基保守性分析以及在葡萄NAC转录因子家族71个成员中进行靶基因预测分析。结果表明:葡萄miR164家族成员的前体序列与双子叶植物聚在一个分支,符合进化规律;葡萄miR164家族4个成员前体序列均可形成稳定的二级茎环结构,成熟序列的碱基保守性较高;葡萄miR164家族成员对应的NAC家族靶基因有2个(GSVIVT01007982001与GSVIVT01020478001),经在NCBI进行BLAST分析,发现均与植物的根发育相关。这暗示着葡萄miR164家族与其靶基因在植物的抗逆过程中发挥重要的调控作用。
MicroRNAs（ miRNAs） play a very important role in regulating the stress resistance and growth development. MiR164 is specific in plants,whose target gene is mainly NAC transcription factor family. By utilizing the bioinformatic methods,we analyzed the phylogenetic of miR164 in Vitis vinifera and some other plants,predicted and analyzed the secondary structure of miR164 family members,the base conservation of the mature sequence,as well as the target gene in NAC（ 71 members） family of Vitis vinifera. The results showed that four members of miR164 in Vitis vinifera were clustered with the dicotyledon plants,which was accorded with evolution law. The precursors of four members could form stable secondary stem-loop structure. Otherwise,the base of mature sequence of four members were highly conserved. According to predicting and analyzing of the target gene,we found two NAC members（ GSVIVT01007982001 and GSVIVT01020478001）. By BLAST in NCBI,it showed that they were related with plant root development,which indicated that miR164 family and their target gene in Vitis vinifera play important regulatory role in root development and stress resistance.
出处
《浙江农业学报》
CSCD
北大核心
2016年第3期441-446,共6页
Acta Agriculturae Zhejiangensis
基金
苏北科技发展计划项目(BN2014101)
关键词
葡萄
miR164
靶基因
抗逆
生物信息学
Vitis vinifera
miR164
target gene
stress resistance
bioinformatics
