摘要
目的观察排毒保肾丸对5/6肾切除大鼠肾纤维化的疗效并探讨其作用机制。方法将50只SD雄性健康大鼠按1∶1∶3比例随机分为正常组(10只)、假手术组(10只)、造模组(30只),假手术组只分离肾包膜不行肾切除,造模组建立5/6肾切除模型。收集大鼠24 h尿液,检测24 h尿蛋白定量(24 h urinary protein,24h UP);采血检测血清尿素氮(blood urea nitrogen,BUN)和肌酐(serum creatinine,SCr)。根据血SCr水平再将30只造模组大鼠随机分为模型组、排毒保肾丸组、尿毒清颗粒组,每组10只。排毒保肾丸组和尿毒清颗粒组分别予排毒保肾丸[1.0 g/(kg·d)]和尿毒清颗粒[3.33 g/(kg·d)]溶于蒸馏水中灌胃,正常组、假手术组、模型组均予2 m L蒸馏水灌胃。均每日1次,共给药4周。观察给药过程中大鼠精神状态、活动度、体毛色泽、大便形状等一般情况和体重的变化。4周后收集大鼠24 h尿液,检测24 h UP;采血检测BUN、SCr、转化生长因子β1(transforming growth fator-β1,TGF-β1)、Ⅲ型前胶原(procollagen typeⅢ,PCⅢ)、Ⅳ型胶原(collagen typeⅣ,ColⅣ)、层粘连蛋白(laminin,LN)、纤维连接蛋白(fibronectin,FN);处死大鼠,取左侧残余肾组织行病理检查,免疫组织化学法检测肾组织TGF-β1、FN蛋白表达量,实时荧光定量PCR检测肾组织TGF-β1、FN mRNA表达。结果与正常组比较,假手术组大鼠上述观察指标差异无统计学意义(P>0.05);与假手术组比较,模型组大鼠一般情况较差,体重增长缓慢,24 h UP增加(P<0.01),血清BUN、SCr、TGF-β1、PCⅢ、ColⅣ、LN、FN升高(P<0.01),残肾病变重,肾组织TGF-β1、FN蛋白及TGF-β1、FN mRNA表达增加(P<0.01);与模型组比较,排毒保肾丸组和尿毒清颗粒组大鼠一般情况较好,体重增长较快,血BUN、SCr下降(P<0.01),24 h UP减少(P<0.05),血TGF-β1、PCⅢ、ColⅣ、LN、FN降低(P<0.05,P<0.01),残肾病变轻于模型组,肾组织TGF-β1、FN蛋白及TGF-β1、FN mRNA表达减少(P<0.01);与�
Objective To observe the anti-renal fibrosis effect of Paidu Baoshen Pill（ PBP） on 5 / 6 nephrectomized rats and to explore its mechanism. Methods Totally 50 SD male healthy rats were randomly divided into the normal control group（n = 10）,the sham-operation group（n = 10）,and the nephrectomy model group（n = 30） according to the proportion of 1∶ 1∶ 3. Rats in the sham-operation group had their renal capsule isolated without nephrectomy. Rats in the nephrectomy model group had their kidneys 5 / 6 nephrectomized. Then 24 h urine was collected and 24 h urinary protein（ 24 h UP） detected. Serum blood urea nitrogen（ BUN） and serum creatitine（ SCr） were also tested. According to the SCrlevel 30 rats of the model group were further randomly divided into the model group,the PBP group,and the Niaoduqing Granule（ NG） group,10 in each group. Rats in the PBP group and the NG group were respectively administered with PBP（ at the daily dose of 1. 0 g/kg） and NG（ at the daily dose of 3. 33 g/kg） by gastrogavage（ they were dissolved in distilled water）. At the same time,2 m L distilled water was administered by gastrogavage to rats in the normal control group,the sham-operation group,and the nephrectomy model group,once daily for 4 successive weeks. Mental conditions,activities,hair color,shape of stool,and the body weight were observed during administration. After 4 weeks,urine was collected to detect 24 h UP. Blood was sampled to detect SCr,BUN,transforming growth factor β1（ TGF-β1）,type Ⅲ procollagen（ PCⅢ）,collagen type Ⅳ（ Col Ⅳ）,laminin（ LN）,and fibronectin（ FN）. After rats were killed,their left remnant renal tissues were collected for pathological examinations. The protein expression quantity of TGF-β1and FN was detected by immunohistochemical method. mRNA expression levels of TGF-β1and FN were detected using real time fluorescent quantitative PCR. Results There was no statistical difference in the above indices between the normal control group a
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2015年第1期81-87,共7页
Chinese Journal of Integrated Traditional and Western Medicine
基金
江西省科技厅科技计划课题(No.20111BBG70008-1)
