摘要
目的观察清肾颗粒对慢性肾功能衰竭(chronic renal failure,CRF)湿热证患者核转录因子(NF-κB)信号转导通路中NF-κB p65、磷酸化NF-κB抑制蛋白(p-IκBα)表达的影响,探讨清肾颗粒治疗CRF患者的作用机制。方法 68例CRF湿热证患者,按随机数字表法分为对照组和治疗组,每组34例,实际完成63例,治疗组32例,对照组31例,并设正常组20名。治疗组与对照组均给予西医基础治疗及中药保留灌肠,治疗组加服清肾颗粒,每日3次,每次1袋,疗程均为8周。观察患者临床疗效、治疗前后血肌酐(SCr)、肾小球滤过率估算值(e GFR)以及外周血中NF-κB p65、p-IκBα的水平变化及其与正常组比较。结果治疗组临床疗效和中医证候疗效总有效率为84.38%(27/32)和81.25%(26/32),优于对照组的54.84%(17/31)和51.61%(16/31),差异有统计学意义(P<0.01)。与本组治疗前比较,治疗后治疗组SCr水平明显下降(P<0.01),e GFR水平明显升高(P<0.01),且优于对照组同期(P<0.05)。与正常组比较,治疗组和对照组治疗前NF-κB p65、p-IκBα水平升高(P<0.01);与本组治疗前比较,治疗后治疗组NF-κB p65、p-IκBα水平均明显降低(P<0.01),且均优于对照组同期(P<0.01)。结论清肾颗粒能改善CRF湿热证患者的临床症状,降低SCr、升高e GFR水平,具有保护肾功能的作用,其作用机制可能与降低外周血中NF-κB p65、p-IκBα水平有关。
Objective To observe the effect of Qingshen Granule （QG） on expressions of nucle- ar factors-KB p65 （NF-KB p65） and phosphonated inhibitor of nuclear factor-KB （ p-IκBα） in peripheral blood NF-KB signal transduction pathway of chronic renal failure （CRF） patients with damp-heat syn- drome （DHS）, and to study possible mechanism. Methods Totally 68 CRF patients with DHS were as- signed to the control group and the treatment group by random digit table, 34 in each group. Actually 63 patients completed, 32 in the treatment group and 31 in the control group. A normal group （20 cases） was set up. All patients received basic treatment of Western medicine （WM） and retention enema of Chi- nese medicine （CM）. Patients in the treatment group additionally took QG, 1 package each time, 3 times per day. The therapeutic course for all was 8 weeks. The clinical efficacy, level of serum creatinine （SCr）, estimated glomerular filtration rate （eGFR）, the levels of NF-KB p65 and p-IκBα in peripheral blood were observed and measured before and after treatment. They were also compared with those of the normal group. Results The clinical efficacy and the total effective rate of CM syndrome were 84.38% （27/32）and 81.25% （26/32）, superior to those of the control group E54.84% （17/31）, 51.61%（16/31）; P 〈0.01 1. Compared with before treatment, the level of SCr was obviously lower, and eGFR was obviously higher in the treatment group after treatment （P 〈0.01 ）. They were better than those of the control group after treatment （P 〈0.05）. Compared with the normal group, the levels of NF-KB p65 and p-IκBαwere significantly higher in the treatment group and the control group before treatment （P 〈 0.01 ）. Compared with before treatment, the levels of NF-KB p65 and p-IκBαwere obviously lowered in the treatment group after treatment （P 〈0.01 ）. They were also better than those of the control group after treatment （P 〈0.05）. Conclusions QG could improve c
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2017年第1期23-27,共5页
Chinese Journal of Integrated Traditional and Western Medicine
基金
国家自然科学基金资助项目(No.81473673
81403372)
安徽省自然科学基金资助项目(No.1508085MH198)
