摘要
为比较玉米黄素和叶黄素单体的体外淬灭单线态氧能力,本实验分别萃取万寿菊与枸杞中的叶黄素与玉米黄素酯,经皂化和纯化后得到游离的叶黄素与玉米黄素组分.使用C30-HPLC-PDA分别对制备组分进行定性和定量分析.用次氯酸钠-双氧水反应产生单线态氧,使用微弱发光测量仪测定制备组分对单线态氧的淬灭率.结果显示：游离叶黄素和玉米黄素均能减少次氯酸钠-双氧水反应体系产生的单线态氧数量,导致最大光子计数值下降.二者淬灭单线态氧的能力相似,最小作用剂量均为0.005～0.05 g/L.
This study aimed to compare the abilities of free zeaxanthin and lutein to quench singlet oxygen in vitro.Lutein and zeaxanthin esters were extracted from marigold flowers and wolfberry fruits,respectively.After being saponified and purified,free lutein and zeaxanthin fractions were obtained from the two extracts,respectively.The fractions were identified and quantified on C30-HPLC-PDA.A reaction between NaClO and H2O2 was then performed to produce singlet oxygen.The singlet oxygen quenching rates of free lutein and zeaxanthin fractions were determined,respectively,on an ultra-weak luminescience analyzer.Data from ultra-weak luminescience analysis suggested that both lutein and zeaxanthin fractions were capable of reducing the amount of singlet oxygen produced and the maximum number of photons generated from the reaction.A minimum amount of 0.005-0.05 g/L that resulted in more than 50％ reduction of maximum photon number was observed with both fractions and their abilities to quench singlet oxygen were similar.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2014年第7期77-80,共4页
Food Science
关键词
玉米黄素
叶黄素
微弱发光分析
单线态氧
自由基
淬灭
zeaxanthin
lutein
ultra-weak luminescience analysis
singlet oxygen
free radical
quenching
