摘要
目的:从酸浆(Physalis alkengi L.var.franchetii(Mast.)Makino)宿萼中制备β-隐黄质(β-cryptoxanthin),应用微弱发光测量方法对β-隐黄质体外淬灭单线态氧的能力进行评价。方法:用正己烷萃取酸浆宿萼中的β-隐黄质酯后经皂化获得单体,使用C18-HPLC-PDA对制备物进行定性和定量。用次氯酸钠-双氧水反应产生单线态氧,使用微弱发光测量仪检测制备组分淬灭单线态氧的能力。结果:经C18-HPLC-PDA鉴定,制备物中的类胡萝卜素主要为β-隐黄质。微弱发光测量仪的数据显示:β-隐黄质的存在能减少次氯酸钠-双氧水单线态氧体系中产生的最大光子计数,且存在量效关系。0.049～0.49g/L的β-隐黄质可减少50%的最大光子计数。
Objective：To assess the ability of β-cryptoxanthin from the calyx of Physalisalkengi L.var.franchetii （Mast.） Makino to quench in vitro singlet oxygen.Methods：β-cryptoxanthin eater was extracted from the calyx of Physalisalkengi L.var.franchetii （Mast.） Makino with n-hexane and then saponified to produce β-cryptoxanthin fraction.The fraction was identified and quantified on C18-HPLC-PDA.Based on the reaction between NaClO and H2O2,singlet oxygen was produced.Singlet oxygen quenching ability of the β-cryptoxanthin fraction was determined on ultra-weak luminescience analyzer.Results：By C18-HPLCPDA,the prepared fraction was identified as β-cryptoxanthin.The data from ultra-weak luminescience analyzer suggested that the β-cryptoxanthin fraction was able to reduce the maximum number of photon generated from the reaction solution in positive correlation with its amount.β-Cryptoxanthin with an amount ranging between 0.049 g/L and 0.49 g/L resulted in a 50% reduction of maximum photon number.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2010年第17期158-161,共4页
Food Science
基金
"十一五"国家科技支撑计划项目(2008BAI58B06)
