摘要
目的:研究玉米黄素对高脂饮食诱导肥胖小鼠肝脏脂质及能量代谢调控的影响。方法:建立高脂饮食诱导C57BL/6J肥胖小鼠模型,饲喂不同剂量玉米黄素(40和20 mg/kg)干预4周,测定其体重、脂肪和肝脏组织质量;对肝脏组织进行HE染色,并检测肝脏组织中脂质代谢相关因子CCAAT/增强子结合蛋白α(C/EBPα)、固醇调节元件结合蛋白-1c(SREBP1-c)、乙酰辅酶A羧化酶(ACC)和脂肪酸转位酶(CD36),能量代谢相关因子过氧化物酶体增殖物激活受体γ辅激活因子1α(PGC-1α)、解偶联蛋白1(UCP1)和沉默信息调节因子2同源蛋白1(SIRT1)的基因表达及其对AMP依赖的蛋白激酶(AMPK)信号通路的影响。结果:与高脂模型组相比,玉米黄素干预能够显著降低体重增加量、肝脏组织质量,降低肝脏组织脂代谢相关基因C/EBPα、SREBP1-c、ACC和CD36的表达,增加UCP1基因表达,低剂量组可显著激活AMPK信号通路。结论:玉米黄素可通过激活肝脏组织AMPK信号通路,下调AMPK靶基因C/EBPα、SREBP1-c、ACC和CD36的脂质合成基因表达水平,提高能量代谢UCP1基因水平,抑制肝脏脂质累积,并改善高脂饮食肥胖小鼠的病理状态。
Objective:To evaluated the regulatory effects of zeaxanthin on lipid and energy metabolism in obese mice.Methods:The obese mice were fed with or without zeaxanthin(40 and 20 mg/kg)for 4weeks,and the body weight,masses of total adipose and liver tissues were detected;Morphological status of liver was observed through HE staining,and the mRNA expression involved in lipid and energy metabolism of liver including CCAAT enhancer binding protein alpha(C/EBPα),Sterol-regulatory Element-binding Protein-1c(SREBP1-c),Acetyl-CoA carboxylase(ACC),fatty acid translocase(CD36),peroxlsome proliferator-activated receptor-γcoactlvator-1α(PGC-1α),uncoupling protein 1(UCP1)and Silence information regulator T1(SIRT1)as well as AMPK signaling pathway were investigated.Results:Compared with the HFD group,zeaxanthin significantly decreased the body weight gain,liver mass,down-regulated the expression of C/EBPα,SREBP1-c,ACC,CD36 and up-regulated UCP1.What’s more,low dose of zeaxanthin treatment reversed the high-fat diet-induced inhibition of Adenosine 5’-monophosphate(AMP)-activated protein kinase(AMPK)phosphorylation in hepatic tissue and alleviated fatty degeneration of liver.Conclusion:Zeaxanthin inhibited the hepatic lipid accumulation and improved the pathological state in high-fat diet induced obese mice by activated the AMPK pathway and down-regulated the expression of C/EBPα,SREBP1-c,ACC,CD36 and up-regulated UCP1 in liver.
作者
刘美宏
刘回民
谢佳函
徐倩
王娇娇
吴熙
潘聪
郑明珠
刘景圣
Liu Meihong;Liu Huimin;Xie Jiahan;Xu Qian;Wang Jiaojiao;Wu Xi;Pan Cong;Zheng Mingzhu;Liu Jingsheng(College of Food Science and Engineering,Jilin Agricultural University,Changchun 130118;National Engineering Laboratory for Wheat and Corn Deep Processing,Changchun 130118;College of Life Science,Jilin Agriculture University,Changchun 130118)
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2019年第12期31-38,共8页
Journal of Chinese Institute Of Food Science and Technology
基金
“十三五”国家重点研发计划(2016YFD0400702)
吉林省“双十工程”重大科技攻关项目(20150201010NY)
吉林省科技创新人才培养计划(20170520076JH)
关键词
玉米黄素
肥胖
高脂饮食
脂质代谢
zeaxanthin
obesity
high fat diet
lipid metabolism
