摘要
目的 探讨视黄醇结合蛋白4(RBP4)在高脂饮食诱导的非酒精性脂肪性肝病(NAFLD)去势小鼠中的表达变化及意义.方法 将30只8周龄雌性C57BL/6小鼠按随机数字表法分为高脂+去势组、去势组和对照组,每组10只;高脂饮食诱导小鼠NAFLD,手术切除双侧卵巢建立去势模型,20周后处死小鼠;称重、收集3组小鼠血清和肝脏组织.HE及油红O染色法观察肝组织形态学改变和肝细胞质内脂质沉积情况,NAS积分和Ishak标准评价肝脂肪变性和纤维化程度;ELISA法检测血清RBP4蛋白表达水平,免疫组织化学染色法观察肝组织RBP4蛋白表达水平,Western blot法检测肝组织RBP4蛋白及脂肪酸合成酶(FAS)、固醇调节元件结合蛋白-1c(SREBP-1c)等肝组织脂质合成代谢相关蛋白相对表达量.结果 与对照组比较,高脂+去势组小鼠体重、肝脏指数及NAS评分均明显升高(均P<0.01),血清和肝组织RBP4及FAS、SREBP-1c蛋白表达水平均明显增加(均P<0.01).结论 RBP4蛋白与高脂饮食诱导的NAFLD严重程度相关,可能通过肝脂质合成代谢途径参与绝经后NAFLD的发生.
Objective To investigate the expression of retinol binding protein 4 (RBP4) in ovariectomized mice with nonalcoholic fatty liver disease (NAFLD). Methods Thirty female C57BL/6 mice were randomly divided into three groups: high fat diet + ovariectomy group(42% high fat diet fed and bilateral oophorectomized), ovariectomy group (bilateral oophorectomized) and control group (control chew and sham surgery). Mice were sacrificed to obtain peripheral blood samples and liver tissue samples. The levels of RBP4 in peripheral blood were detected by ELISA assay. The expression of RBP4 and related cytokine proteins in liver tissues were analyzed by Western blot and immunohistochemistry. Results Histopathological examination showed that the liver steatosis was most severe in the high fat diet + ovariectomized group, with the significant elevation of the expression of RBP4 protein in liver and serum samples compared to the other two groups(both P<0.01). Conclusion The study indicates that RBP4 may be involved in the pathological process of postmenopausal nonalcoholic fatty liver disease.
作者
蔡泓
卢莎
陈岳明
卓广超
张治芬
CAI Hong;LU Sha;CHEN Yueming(Affiliated Hangzhou First People’s Hospital,Zhejiang University School of Medicine,Hangzhou 310006,China)
出处
《浙江医学》
CAS
2019年第20期2142-2146,I0003,共6页
Zhejiang Medical Journal
基金
浙江省科技厅重大科技专项重点社会发展项目(2014C03044-1)
