摘要
目的观察苦参碱对体外培养的乳腺癌4T1细胞增殖及凋亡的影响,并探讨其可能的作用机制。方法体外培养乳腺癌4T1细胞,通过不同剂量的苦参碱(0. 1 m M、0. 2 m M、0. 4mM)进行干预后,用CCK-8法、流式细胞术检测其对乳腺癌4T1细胞抑制率和凋亡率的影响;实时荧光定量PCR检测其对4T1细胞膜联蛋白A3mRNA表达的影响;蛋白免疫印迹法检测其对4T1细胞膜联蛋白A3蛋白表达的影响。结果苦参碱处理4T1细胞后,各组细胞的OD值均显著降低(P <0. 05),细胞的凋亡率显著升高(P <0. 05);膜联蛋白A3(Annexin A3,ANXA3) mRNA表达明显降低,膜联蛋白A3蛋白表达明显降低,ANXA3 mRNA及蛋白表达趋势相一致(P <0. 05)。结论苦参碱可通过抑制ANXA3的表达,抑制4T1细胞的增殖和促进其凋亡。
Objective To observe the effect of matrine on proliferation and apoptosis of breast cancer 4T1 cells and explore its mechanism. Methods Breast cancer 4T1 cells were cultured in vitro and intervented with different concentrations of matrine ( 0. 1,0 . 2, 0. 4 and 0. 8 mM/L,respectively) . The effects of matrine on the proliferation rate and apoptosis rate of 4T1 cells were detected by method CCK - 8 and Flow cytometry. Real - time fluorescence quantitative PCR was used to detect the expression of annexin A3 mRNA in 4T1 cells. The effect of matrine on the expression of annexin A3 protein in 4T1 cells was detected by Western blotting. Results After treatment with matrine,the OD value of 4T1 cells was significantly decreased ( P< 0.05) ,the apoptosis rate was significantly increased (P < 0.05) ,the relative expression of annexin A3 mRNA and annexin A3 protein was significantly decreased,and the expression trend of ANXA3 mRNA and protein was consistent( P < 0.05) . Conclusion Matrine can regulate the proliferation and apoptosis of 4T1 cells by inhibiting the expression of ANXA3 mRNA and down - regulating the expression of ANXA3 protein.
作者
石文达
李代晓
胡久丽
王莹
杨宁
肖旭
Sill Wenda;LI Daixiao;HU Jiuli;WANG Ying;YANG Ning;XIAO Xu(The Affiliated Hospital of Chengde Medical College,Chengde 067000,China)
出处
《宁夏医学杂志》
CAS
2018年第11期967-969,共3页
Ningxia Medical Journal
基金
承德市科学技术研究与发展计划项目(201701A068)
