摘要
目的探讨苦参碱(matrine,MAT)对人卵巢恶性畸胎瘤细胞株PA-1体外增殖的影响。方法体外培养人卵巢恶性畸胎瘤细胞株PA-1,分组予不同浓度苦参碱作用。MTT法检测0.25、0.50、1.0 mg/mL不同浓度苦参碱对细胞增殖的抑制作用以及药物作用的量效和时效关系;流式细胞仪检测细胞凋亡和细胞周期;半定量RT-PCR检测bcl-2/bax mRNA表达水平的变化。结果MTT法显示药物作用后,细胞有明显的抑制增殖作用,且呈时间依赖性和剂量依赖性;流式细胞仪检测不同浓度的苦参碱作用后PA-1细胞的凋亡率依次上升,并与作用时间正相关;细胞周期结果显示苦参碱对PA-1细胞具有明显的G_1期阻滞作用,且具有剂量依赖性。结论苦参碱能使PA-1细胞bcl-2 mRNA和bax mRNA的比值下调,产生G_1期阻滞,从而调节细胞周期,最终导致PA-1细胞在体外的增殖受到明显抑制。
Objective To study the effect of matrine （MAT） on the proliferation of human ovary malignant teratoma cell line PA-1 in vitro. Methods PA-1 cells allocated in different groups were treated with different concentrations （0.25 mg/mL, 0. 5 mg/mL and 1.0 mg/mL） of MAT. The inhibitory effect of MAT and its doseand time-effect relationship were detected with MTT; the apoptosis rate and cell cycle were evaluated by flow cytometry; and the changes of bcl-2/bax mRNA expression in cells were measured using semi-quantitative RTPCR. Results After being exposed to MAT, the PA-1 cell proliferation was decreased in a concentration- and time-dependent manner; cell apoptosis rate raised as the increasing concentration of MAT and acting time; cells retarded at G1 phase in the cell cycle dose-dependently; and the bcl-2/bax mRNA expression in cells dawn-regulated significantly. Conclusion MAT can dose- and time-dependently inhibit the proliferation of PA-1 cell by reducing bcl-2/bax mRNA ratio to produce a G1 phase arresting in cell cycle.
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2010年第7期723-725,共3页
Chinese Journal of Integrated Traditional and Western Medicine
基金
浙江省自然基金资助项目(No.Y204463)
浙江省卫生厅重点项目(No.2004ZD009)
