摘要
背景:磁性微粒作为一种磁性载体在固定化酶、免疫检测、靶向载药治疗及细胞分离等生物医学领域得到了广泛的应用。目的:制备分散稳定性好,相对磁性强的纳米级Fe3O4微粒。方法:以氯化亚铁、氯化铁、氢氧化钠为主要原料,采用化学共沉淀法合成Fe3O4磁性粒子。结果与结论:用正交设计法优化了Fe3O4微粒的合成工艺条件,得到制备Fe3O4粒子的最佳实验条件为Fe2+/Fe3+的物质的量之比为2∶1、共沉淀时的pH值为11、熟化温度为90℃、表面活性剂聚乙二醇的用量为40mL,此时制得的Fe3O4粒子粒径最小,为78nm,Fe3O4溶液的分散稳定性最好,相对磁性最强。从Fe3O4的扫描电镜图可以看出,Fe3O4微粒晶体颗粒为纳米级。
BACKGROUND：As a kind of magnetic carrier,magnetic nanometer particles have been used in enzyme immobilization,immunoassay,target drug delivery and cell sorting and so on. OBJECTIVE：To prepare nanometer Fe3O4 crystal particles with good dispersion stability and relatively strong magnetic. METHODS：Fe3O4 magnetic particles was synthesized by the chemical co-precipitation,using FeCl2,FeCl3 and NaOH. RESULTS AND CONCLUSION：The synthetic conditions of Fe3O4 magnetic particles were determined through orthogonal design and the optimum experimental conditions as follows：nFe2＋/nFe3＋ was 2：1,pH was 11,curing temperature was 90 ℃ and the amount of PEG was 40 mL. Under this condition,the average diameter of Fe3O4 particles was 78 nm,the dispersion stability was the best and the relative size of magnetic was the strongest. It could be seen from scanning electron microscope that Fe3O4 crystal particles were nanometer.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2011年第34期6385-6387,共3页
Journal of Clinical Rehabilitative Tissue Engineering Research
