摘要
脂肪氧合酶是一种过氧化物酶,具有多种用途,运用合适的方法和材料将脂肪氧合酶固定化,可以提高酶的稳定性、增加使用次数。该试验采用水热法制备纳米Fe3O4,并用SiO2和KH550对颗粒表面进行表面修饰得到复合载体,以戊二醛为交联剂将脂肪氧合酶固定在复合载体上;考察不同因素对游离酶和固定化酶活性的影响。结果表明,微粒在电镜观察下呈亮黑色短棒状粒子,固定在载体上酶的含量约为14%;游离酶的活力为1.82×103 U/mL,最适温度为30℃,最适pH 8.0;而固定化酶的活力为2.31×103 U/g,最适温度为25℃,最适pH 9.0;H2O2浓度为12.0g/L时,游离酶和固定化酶的活性最强。说明以纳米磁性四氧化三铁材料固定脂肪氧合酶是一种可行的方法,具有进一步深入研究的意义。
Lipoxygenase which is a kind of peroxidase could be used in many facets of industry. The stability and using times of the enzyme would be increased after the enzyme was immobilized by appropriate methods and materials. In this study, nanometer Fe304was prepared by hydrothermal method and modified with SiOz and KH550 to ob- tained composite carriers. Lipoxygenase was immobilized on the com- posite carriers by glutaraldehyde. The influence factors on enzyme activity for free and immobilized enzyme were studied. The TEM re- sults showed that the black nanoparticles were rod shaped. The mass of enzyme immobilized on the composite carriers was about 14% ofthe immobilized enzyme mass. The activity of free enzyme was 1.82 × 10^3 U/mL while its optimum temperature and pH were 30 ℃ and 8.0, respectively. The activity of immobilized enzyme was 2. 31 × 10^3 U/g while its optimum temperature and pH were 25 ℃ and 9.0 respectively. The free and immobilized enzyme exhibited the highest enzyme activity when H2O2 concentration was 12. 0 g/L. The results indicated that it was a feasible method to immobilize lipoxygenase by nanometer Fe3O4, and it was deserved to be further studied.
出处
《食品与机械》
CSCD
北大核心
2015年第4期1-4,共4页
Food and Machinery
基金
西华大学2014年校重点科研基金项目(编号:z1420523)
四川省教育厅一般项目(编号:15ZB0126)
关键词
纳米磁性微粒
纳米FE3O4
固定化
脂肪氧合酶
酶活
magnetic nanometer particle
nanometer Fe3O4
immobi- lization
lipoxygenase
enzyme activity
