摘要
研究了一种基于磁性纳米颗粒的日本血吸虫荧光免疫分析方法。日本血吸虫抗原通过共价吸附到核壳结构的磁性颗粒表面,与待测抗体结合后,再与酶标二抗夹心反应,最后以3,3′,5,5′-四甲基联苯胺（TMB）为底物,通过测定酶催化下TMB氧化生成无荧光的二聚体化合物,使溶液荧光强度降低来间接测定日本血吸虫抗体的浓度。应用本方法测定了兔血清中日本血吸虫抗体,荧光强度（If）与抗体浓度（C）在5.0-100μg/L之间呈线性关系,线性回归方程为If=225.8-1.6C（r=0.9976）,检出限达1.5μg/L。方法简单实用,具有良好的检测灵敏度和重现性。
A fluorescence immunoassay using magnetic nanoparticles as the immobilization substrate was proposed for Schistosoma japonicum antibodies （SjAb）. The SjAb in serum samples reacted with the bound Schistosoma japonicum antigens （SjAg） on the magnetic particles of core-shell structure, and the horserash peroxide labeled secondary. Antibodies were then bound to the magnetic particles by a sandwich reaction. Using 3,3′,5,5′-tetramethyl benzidine （TMB） as a fluorescent substrate, the SjAb was determined indirectly by measuring the decrease in fluorescent intensity resulting from the catalytic oxidation of TMB. Using the fluorescence immunoassay method, the fluorescence intensity （If） of the TMB substrate solution is linearly related to the SjAb concentration （C） in rabbit serum in the range of 5.0 -100 μg/L with a regression equation of If =225.8 - 1.6C（r =0. 9976） , and the detection limit reaches 1.5 μg/L, which shows good detection sensitivity.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2008年第11期1455-1459,共5页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金(No.20205004)
教育部科学技术研究重点项目(No.106122)资助
