摘要
采用具有较高操作稳定性的交联β-葡萄糖苷酶聚集体,在离子液体-混合有机溶剂-缓冲溶液的非常规反应体系中酶催化直接糖基化合成红景天苷,筛选反应体系的组成和各成分的比例.结果表明,反应体系的组成为(%,φ):1,4-二氧六环42.5,离子液体C4MIm·PF6 8.5,乙酸乙酯34,p H 6.0柠檬酸-磷酸氢二钠缓冲溶液15.0.该体系在水含量高达15%(φ)的条件下水活度为0.66,适合直接糖基化反应.底物β-D-葡萄糖100 g/L、对羟基苯乙醇200 g/L时,在50℃及250 r/min下反应120 h达到平衡,红景天苷最大浓度可达25.32 g/L.
In a non-traditional reaction system with ionic liquid, mixed organic solvents and buffer solution, synthesis of salidroside with highly operation stable cross-linked β-glucosidase aggregates by direct glycosylation under enzymatic catalysis was studied. Based on the optimization of the composition of reaction media and the proportion of each component, the reaction system was determined as follows in volume ratio： 8.5% of ionic liquid CaMIm.PF6, 42.5% of 1,4-dioxane, 34.0% of ethyl acetate, and 15.0% of citric acid-disodium hydrogen phosphate buffer （pH 6.0）. In this reaction system, the water activity was maintained at the proper level which was suitable to the direct glycosylation even though the water content of the system was up to 15%. So, at the reaction temperature of 50 ℃ and the rotating speed of 250 r/rain, when 13-D-glucose content was 100 g/L and p-hydroxyphenylethanol content 200 g/L, the equilibrium concentration of salidroside could reach up to 25.32 g/L after reaction for 120 h.
出处
《过程工程学报》
CAS
CSCD
北大核心
2015年第2期313-317,共5页
The Chinese Journal of Process Engineering
基金
国家海洋公益性行业科研专项基金资助项目(编号:201205022-3)
