摘要
目的:观察不同剂量斑蝥酸钠在体外作用24h对人肝癌细胞HepG2生长的影响。方法:用MTT法及AO/EB荧光染色法分析斑蝥酸钠对HepG2的生长抑制情况。结果:小、中、大剂量斑蝥酸钠作用24h,对HepG2的抑制率分别为0、24.37%、25.54%,大剂量有明显抑制作用(P<0.01);荧光染色法显微镜下可观察到凋亡小体。结论:斑蝥酸钠能抑制肝癌细胞生长,并且可诱导其凋亡。
Objective：To study different dosage of sodium cantharidate＇s inhibitory effect on the human hepatocarcinoma cell HepG2 at 24h. Methods：The inhibitory effect of HepG2 treated by sodium cantharidate was analyzed by MTT,and fluorescent microscope. Results：The inhibitory rate of three dosages of sodium cantharidate on HepG2 was 0,24.37%,25.54% at 24h,and this has statistical acceptation（P0.05）,and we could observe the phenomena of budding and the formation of the apoptosis body by fluorescent microscope. Conclusions：Sodium cantharidate can inhibit cell proliferation and induce cell apoptosis of HepG2.
出处
《辽宁中医药大学学报》
CAS
2010年第2期41-42,共2页
Journal of Liaoning University of Traditional Chinese Medicine
基金
江苏省重点实验室开放基金资助项目
关键词
斑蝥酸钠
抑制率
肝癌
Sodium cantharidate inhibitory effect human hepatocarcinoma
