摘要
目的:从中药中筛选有抗肿瘤活性的化合物。方法:应用人乳腺癌细胞系BCAP细胞筛选26种生物碱类化合物对其增殖的抑制作用。结果:小檗胺和喜树碱对人乳腺癌细胞株BCAP细胞增殖有一定程度的抑制作用;其次为马兜铃酸I和利血胺;西萝芙木碱、番茄碱和那可丁仅有较弱的抑制活性;它们的抑制活性皆呈浓度-效应关系。士的宁、β-可鲁勃林、依卡金、萝芙木辛、育亨宾、柯楠碱、蛇根精、长春胺、骆驼蓬碱、茵芋碱、白藓碱、γ-崖椒碱、4-甲氧基-1-甲基-2-喹诺酮、去氢贝母碱N-氧化物、倒千里光碱、毛果芸香碱、DL-N-甲基四氢罂粟碱、左旋麻黄碱和左旋去甲基伪麻黄碱在本实验条件下无效。结论:小檗胺、喜树碱、马兜铃酸I、番茄碱和利血胺可能是含有该生物碱的、有抗肿瘤活性中药的有效成分,亦可能成为开发抗肿瘤新药的候选药物。
Objective： To screen antitumor active compounds from traditional Chinese materials medica. Methods： Twenty -six alkaloids compounds isolated from the traditional Chinese materials medica were studied for their antitumor activity in vitro by determining the inhibitory percentage against growth of human mammary cancer cell line BCAP. Results： It showed that berbamine, camptothecin, aristolochic acid Ⅰ, rescinnamine, ajmaline, tomatidine and noscapine inhibited growth of human mammary cancer cell line BCAP cell and their activity showed dose -effect relationship, which the inhibitory effects of berbamine and camptothecin were stronger more than aris- tolochic acid I and rescinnamine, and ajmaline, tomatidine and noscapine were weaker. However, strychnine, β- colubrine, icajine, ajmalicine, yohimbine, corynanthine, sarpagine, vincamine, harmaline, skimmianine, dictamnine, γ- fagarine, 4 - methoxy - 1 - methyl - 2 - quinolone, verticinone N - oxide, retrorsine, pilocarpine, DL - laudanosine, （ - ） - ephedrine and （ - ） - norpseudo - ephedrine did not inhibit the growth of human mammary cancer cell line BCAP in this assay condition. Conclusion： Berbamine, camptothecin, aristolochic acid Ⅰ, tomatidine and rescinnamine are likely to be effective constituents in corresponded traditional Chinese materials medica to inhibit growth of tumor cells or regarded potent candidating or leading compounds for research and development of anti - tumor drugs.
出处
《中国现代中药》
CAS
2007年第3期9-11,共3页
Modern Chinese Medicine
基金
科技部新医药博士基金资助项目
关键词
中药
生物碱类
人乳腺癌细胞株BCAP
Traditional chinese materials medica
Alkaloids
Mammary cancer cell line BCAP
