摘要
应用免疫组织化学抗生蛋白链菌素———生物素标记法（ＬＳＡＢ法），对１２２例乳腺癌ｎｍ２３基因的表达及其与淋巴结转移的关系进行了研究。结果显示：１２２例乳腺癌ｎｍ２３基因阳性率为５１．６％（６３／１２２），而有腋窝淋巴结转移的５３例乳腺癌，其阳性率为２８．３％（１５／５３），无腋窝淋巴结转移的６９例，阳性率为６９．６％（４８／６９），两者有非常显著差异（Ｐ＜０．０１）。同时发现ｎｍ２３基因表达与乳腺癌组织学分类、肿瘤大小、年龄无相关性。提示ｎｍ２３基因不仅在乳腺癌中具有高表达，而且在乳腺癌淋巴结转移中起重要作用。因此，检测ｎｍ２３基因表达可作为临床预测乳腺癌淋巴结转移趋势的重要参考指标之一，对判断预后和制定治疗方案具有参考价值。
Using labelled streptavidin biotin (LSAB) method, we examined the relationship between expression of nm23 and lymph node metastasis in 122 human primary breast cancers. Of 122 breast cancer patients, in 63(51.6%) cases nm23 gene expressed positively.The positive staining rate was higher in without axillary lymph node metastasis (69.6%) than that in patients with axillary lymph node metastasis (28.3%)( P <0.01). Expression of nm23 gene was not correlated with tumor histological type, tumor size and age of patients ( P >0.05). The results suggest that the expression of nm23 gene is higher in human primary breast cancer, and play an important role in lymph node metastasis of breast cancer. Therefore, detection of nm23 expression may be of significant clinical importance in the evaluation of the tendency of lymphatic metastasis and be helpful to calculate the prognosis and work out the theerapeutic regimen of breast cancer.
出处
《实用癌症杂志》
1997年第1期9-11,共3页
The Practical Journal of Cancer
关键词
乳腺肿瘤
NM23
淋巴转移
免疫组织化学
Breast neoplasm nm23 gene Lymphatic metastasis Immunohistochemistry
