摘要
应用免疫组织化学ＳＰ方法，研究６４例乳腺癌手术标本中ｎｍ２３的表达及其预后意义。结果显示：原发灶癌组织中ｎｍ２３高表达的乳腺癌，区域淋巴结转移发生率明显低于低表达者（Ｐ＜０．０１）；组织学高分化的乳腺癌或肿瘤最大径≤２ｃｍ者，原发灶ｎｍ２３表达水平较高（均Ｐ＜０．０５）；随着临床病理分期的进展，乳腺癌原发灶中ｎｍ２３表达水平逐渐下降（Ｐ＜０．０１）；高表达的患者术后３年、５年生存率分别６９．４％和５８．７％，明显高于低表达者（４２．９％和２５．０％），Ｐ＜０．０５和Ｐ＜０．０１。以上结果表明，ｎｍ２３在乳腺癌淋巴结转移过程中发挥负性调控作用，并与乳腺癌的组织学分化有关。
In resected specimens of breast carcinoma the levels of nm23 were examined immunohistochemically, using anti-nm23 polyclonal antibody.Of 64 tumors tested,36(56.25%)showed strong immunoreactivity for nm23,while others(43.75%)showed low or no immunoreactivity.strong nm23 immunoreactivity was found to be associated with lower incidence of lymph node metastasis(P<0.01),or well-differentiated tumors(P<0.05).Inverse relationships were observed between nm23 expression and tumor size(P<0.05)or the clinicopathological stage(P<0.01).Furthermore, the intensity of nm23 expression showed positive correlation with longer post-operative survival(P<0.01).Our data indicated that nm23 gene plays a role in suppressing the metastatic phenotype in breast carcinoma.Detection of nm23 expression may be useful for the prognosis of breast carcinoma.
关键词
基因
免疫组织化学
乳腺癌
预后
nm23 gene
immunohistochemistry
breast carcinoma
