摘要
目的 :探讨胃癌发生中CyclinB1、CDK1表达异常及其临床病理学意义。方法 :采用免疫组织化学ABC法检测CyclinB1、CDK1在 15例正常胃组织、35例胃癌组织 (其中 15例为低分化腺癌 ,2 0例为中、高分化腺癌 )中的表达。结果 :CyclinB1、CDK1阳性表达多数定位于细胞质 ,少数在细胞核和细胞质中同时表达 ;在胃正常粘膜中CyclinB1、CDK1表达阳性率分别为 13.3% (2 / 15 )、6 .7% (1/ 15 ) ,胃癌组织中表达阳性率分别为 94 .3% (33/ 35 )、91.4 % (32 / 35 ) ,有统计学意义 (P <0 .0 1) ;在低分化腺癌、中高分化腺癌CyclinB1、CDK1均有表达 ,不同分化程度胃癌之间阳性表达差异有统计学意义 (P <0 .0 1)。结论 :CyclinB1、CDK1在不同分化程度胃癌中有过表达现象 ,可能是胃癌发生中早期分子事件 。
Objective:To investigate the clinicopathological significance of the CyclinB1 and CDK1 expression in stomach carcinogenesis.Methods:The expression of CyclinB1 and CDK1 was examined by immunohistochemistry method in normal epithelium(15 cases),low-differentiated adenocarcinoma (15 cases)and moderate-well-differentiated adenocarcinoma (20 cases).Results:Immunoreaction to Cyclin B1 and CDK1 was mainly localized in the cytoplasm of cells.In stomach carcinogenesis,the positive immunostaining rate of CyclinB1 protein in normal epithelium and adenocarcinoma was 13.3%(2/15),94.3%(33/35) respectively (P<0.01),while CDK1 protein was 6.67%(1/15),91.4%(32/35) (P<0.01).There was distinct difference of CyclinB1 and CDK1 expression in the vary state of stomach adenocarcinoma(P<0.01).Conclusion:CyclinB1 and CDK1 could be an early molecular event in the carcinogenesis of stomach and may play an important role in stomach carninogenesis.
出处
《中国现代普通外科进展》
CAS
2004年第5期272-274,共3页
Chinese Journal of Current Advances in General Surgery
基金
国家自然科学基金B类 ( 3 0 2 2 80 3 1)
