摘要
目的观察CD174、cyclinD1和cyclinE在消化道肿瘤组织的表达水平,以及三者之间的相关性,初步探讨CD174对消化道肿瘤诊断和预后判定的价值。方法用流式细胞仪分别检测消化道肿瘤组织及相应的远端组织中CD174、cyclinD1和cyclinE蛋白质的表达。用免疫组化染色了解消化道组织中CD174的表达和分布状况。结果CD174在消化道肿瘤组织的表达水平明显低于瘤旁正常组织(P<0.05),cyclinD1和cyclinE表达水平则高于瘤旁正常组织(P<0.05)。CD174、cyclinD1和cyclinE在不同性别人群中的表达无统计学意义(P>0.05);cyclinE的表达水平在肿瘤长径>3cm组明显高于肿瘤长径≤3cm组(P<0.05);CD174在腺癌中表达水平高于鳞癌(P<0.05);CD174和cyclinE蛋白质的表达水平在TNM分期为Ⅲ～Ⅳ期肿瘤组织中低于Ⅰ～Ⅱ期肿瘤(P<0.05),而cyclinD1表达水平则相反(P<0.05)。肿瘤组织中CD174与cyclinD1和cyclinE蛋白质呈负相关(r=-0.61、r=-0.57)。免疫组化染色亦显示,肿瘤组织中CD174阳性细胞率明显低于瘤旁组织。结论CD174在消化道肿瘤组织的表达水平明显降低,且与肿瘤的分化、浸润和转移相关。CD174与cyclinD1、cyclinE的联合检测有助于消化道肿瘤的诊断和预后的判断。
Objective To observe the expression levels of CD174, cyclin D1 and cyclin E in gastrointestinal tumor tissues, analyze the correlation among CD174, cyclin D1 and cyclin E, and evaluate the expression level of CD174 for diagnosis and prognosis of gastroin- testinal tumors. Methods Flow cytometry was used to determine the expression of CD174, cyclin D1 and cyclin E on surface of the cells which were collected from the tumor tissues and distant part beside the same tumor and suspended. The expression and topography of CD174 in gastrointestinal tissue were investigated with immunohistochemical staining. Results The expression of CD174 in tumor tissues was obviously lower than that in distal noncancerous tissues （P 〈 0.05 ） while the expressions of cyclin D1 and cyclin E were higher. In contrast to the clinical and pathological conditions, the expressions of CD174, cyclin D1 and cyclin E had no correlation with gender. The expression level of cyclin E in the cells of big tumor （ diameter more than 3 cm） was obviously higher than that of small tumor （diameter less than 3 cm）. The expression level of CD174 in the cells of adenocarcinoma was lower than that in squamous carcinoma （P 〈0.05 ）. The expression levels of CD174 and cyclin E in the tumors with TNM stage Ⅲ or IV were significantly lower than those with TNM stageI or Ⅱ tumors （ P 〈 0.05 ） , while the expression of cyclin D1 was opposite （ P 〈 0.05 ）. The expression of CD174 had negatively correlation with cyclin D1 and cyclin E in tumor tissues （r = -0.61 and r = -0.57 ）. The results of immunohistochemical staining showed that the CD174-negative cells made up of the majority in the distal noncancerous tissues but the majority of cells in tumor tissues was CD174-positive. Conclusions The expression of CD174 was notably low in the tissues of gastrointestinal tumor and correlated with differentiation, infiltration and metastasis of the tumor. The combined detection of CD174, cyclin D1 and cyclin E should be helpful to diagnose
出处
《临床检验杂志》
CAS
CSCD
北大核心
2007年第6期435-437,共3页
Chinese Journal of Clinical Laboratory Science
