摘要
目的探讨p53Arg型和p53Pro型蛋白功能失活与细胞周期调控异常在HPV相关子宫颈鳞癌发生机制中的作用。方法应用免疫组织化学方法检测p53、CyclinD1、Cdk4、Rb磷酸化及Ki-67蛋白表达(PI)。结果在HPV阳性p53蛋白阴性的子宫颈鳞癌组中CyclinD1、Cdk4蛋白表达、Rb蛋白磷酸化率及PI值均明显高于子宫颈对照组(χ2=16.878,P<0.01;χ2=21.120,P<0.01;χ2=40.36,P<0.01;t=30.53,P<0.01)。CyclinD1与Cdk4蛋白表达、Rb磷酸化率均呈正相关(P<0.01),Rb高磷酸化组中的PI值高于低磷酸化组(P<0.05)。p53Pro型子宫颈鳞癌组中CyclinD1蛋白表达和PI值均高于p53Arg型组和p53Pro/Arg型组(P<0.05)。结论 HPV阳性的子宫颈鳞癌组中部分p53蛋白表达阴性,提示该部分p53蛋白被E6蛋白降解。在p53阴性的子宫颈鳞癌组织中PI值增高与CyclinD1-Cdk4-Rb磷酸化途径有关。p53Pro型组比p53Arg型组蛋白可能具有较强的细胞周期阻滞功能。
Objective To study the role of inactivation of p53Arg protein and p53Pro protein and aberrant regulation of cell cycle in the mechanism of HPV-related cervical cancer.Methods The expression of CyclinD1,Cdk4,phosphorylation of Rb and Ki-67（PI） were detected by immunohistochemistry.Results The expressions of CyclinD1,Cdk4,high phosphorylation rate of Rb and PI values in cervical cancer of p53 negative group were higher than those in the control group（χ2=16.878,P〈0.01;χ2=21.120,P〈0.01;χ2=40.36,P〈0.01;t=30.53,P〈0.01）.The expressions of CyclinD1 and Cdk4,phosphorylation of Rb showed a positive correlation（P〈0.01）.The PI values in phosphorylation Rb positive group was higher than that in the negative group（P〈0.05）.The expression of CyclinD1 and PI values of p53Pro genotype were higher than those of the p53Arg and p53Pro/Arg genotype（P0.05）.Conclusions HPV positive cervical cancer shows part of p53 protein are negatively expressed,which indicates that the p53 protein is degraded by E6 protein.The high PI values in the p53 protein negatively expressed cervical cancer tissues is related to the CyclinD1-Cdk4-Rb pathway.p53Pro protein has stronger abiliy to arrest cell cycle than p53Arg protein.
出处
《实用肿瘤杂志》
CAS
北大核心
2011年第1期17-21,共5页
Journal of Practical Oncology
基金
国家自然科学基金资助项目(30560046)
