摘要
目的 探讨胃癌组织中细胞周期素E(cyclinE)及细胞周期素依赖性激酶 2 (CDK2 )的表达与胃癌临床病理因素的关系。方法 应用蛋白质印迹法测定 36例胃癌组织标本及其相应的正常胃粘膜中cyclinE及CDK2的表达情况。结果 胃癌组织中cyclinE及CDK2表达水平均明显高于正常胃粘膜 (P <0 0 5 )。在低分化、有淋巴结转移和 (或 )远处转移及浸润胃壁全层的胃癌中cyclinE表达水平明显增高 (P <0 0 5 ) ,在有淋巴结转移和 (或 )远处转移的胃癌中CDK2表达水平明显增高 (P <0 0 5 )。结论 CyclinE及CDK2的表达水平与胃癌的恶性潜能有关 。
Objective To determine the relationship between the expression of cyclin E and cyclin dependent kinase 2(CDK2) protein and malignant potentials in human gastric cancer.[WT5”HZ] Method [WT5”BZ]Samples from 36 gastric cancer patients undergoing surgery were studied, the protein expression of cyclin E and CDK2 in gastric cancer and adjacent normal mucosa were measured by Western blot.[WT5”HZ] Result [WT5”BZ] The protein levels of cyclin E and CDK2 in tumor tissue were higher than that in adjacent normal mucosa( P <0 05).It was found that over expression of cyclin E was related to regional lymph nodes metastasis, poor tumor differentiation and serosal invasion in gastric cancer ( P <0 05). Over expression of CDK2 was related to regional lymph nodes metastasis( P <0.05). [WT5”HZ] Conclusion [WT5”BZ] The cyclin E CDK2 complex may be related to the malignant transformation, development and metastasis of gastric cancer by promoting cell proliferation.
出处
《中华普通外科杂志》
CSCD
北大核心
2001年第5期301-303,共3页
Chinese Journal of General Surgery
基金
中华医学基金项目!(ORF980 7)
教育部留学回国启动基金项目!(F438)
