摘要
目的:观察MTA1,PTEN,E-cadherin蛋白在胃癌和正常胃黏膜组织中的表达,探讨其与胃癌浸润、转移和生物学行为的关系．方法:应用免疫组织化学方法检测54例胃癌手术切除标本和15例正常胃黏膜组织中MTA1,PTEN,E-cadherin的表达．各指标之间相关因素的差异性比较采用x2检验,相关性研究采用Spearman相关分析:结果:与正常胃组织相比,MTA1蛋白在胃癌组织中高表达(46．3％vs6．7％,P<0．01),PTEN和E-cadherin蛋白在胃癌组织中表达下调或缺失(51．9％vs 100％,42．6％vs 100％,均P<0．01)．MTA1和PTEN的阳性表达率与肿瘤浸润深度(P=0．003,P=0．001)、病理分期(P=0．004, P=0．008)、淋巴转移(P=0．000,P=0．001)、远隔转移(P=0．004,P=0．006)、临床分期有关(P=0．001,P=0．000);E-cadherin的正常表达率与肿瘤浸润深度(P=0．027)、病理分化程度(P=0．006)、淋巴转移(P=0．044)、临床分期有关(P=0．000)．Spearman相关分析显MTA1与PTEN蛋白、MTA1与E-cadherin蛋白的表达呈负相关(r=-0．518,r=-0．424,均P<0．05)．PTEN蛋白与E-cadherin蛋白的表达呈正相关(r=0．53,P<0．05)．结论:MTA1蛋白水平高表达和PTEN, E-cadherin蛋白水平低表达可能与胃癌浸润和转移有关,且联合检测可以用于判断胃癌的生物学行为．
AIM： To investigate the expression of metastasis- associated gene 1 （MTA1）, PTEN and E-cadherin in gastric carcinoma and normal gastric mucosa and their relationships with the invasion, metastasis and biological behavior of gastric carcinoma.
METHODS： Immunohistochemistry was used to examine the expression of MTA1, PTEN and E-cadherin proteins in gastric carcinoma （n =54） and normal gastric mucosa （n = 15）. The data were processed by chi-square test and the correlation was analyzed by Spearman test.
RESULTS： In comparison with those in normal gastric mucosa, the expression of MTA1 in gastric carcinoma was significantly higher （46.3% vs 6.7%, P 〈 0.01）, and the expression of PTEN and E-cadherin in gastric carcinoma were downregulated or even disappeared （51.9% vs 100%,42.6% vs 100%; both P 〈 0.01）. The expression of MTA1 and PTEN were correlated with the invasion depth （P=0.003, P = 0.001）, pathological grades （P = 0.004, P = 0.008）, lymph node metastasis （P = 0.000, P = 0.001）, distant metastasis （P = 0.004, P=0.006） and clinical classification （P= 0.001, P = 0.000）, and the normal expression of E-cadherin had relationship with the invasion depth （P = 0.027）, pathological grades （P = 0.006）, lymph node metastasis （P = 0.044）, and clinical classification （P = 0.000）. There was a negative correlation between the expression of MTA1 and PTEN as well as between the expression of MTA1 and E-cadherin （r = -0.518, r = -0.424; both P 〈 0.05）, but there was a positive correlation between the expression of PTEN and E-cadherin （r = 0.53, P 〈 0.05）.
CONCLUSION： High expression of MTA1 and low expression of PTEN and E-cadherin may be associated with the invasion and metastasis of gastric carcinoma. Combined examination of three indexes may be used to evaluate the biological behavior of gastric carcinoma.
出处
《世界华人消化杂志》
CAS
北大核心
2007年第10期1096-1102,共7页
World Chinese Journal of Digestology
