摘要
目的 探讨以小鼠骨髓基质干细胞 (MSCs)作为组织工程软骨的种子细胞 ,在人耳脱细胞软骨支架上生长的可行性。 方法 将人耳软骨经脱细胞处理 ,得到脱细胞软骨支架。抽取鼠的骨髓 ,经离心得到单个核细胞 ,进行体外分离培养得到 MSCs。将鼠的第 2代 MSCs种植于脱细胞软骨支架上 ,体外立体培养 10天 ,在光镜及电镜下观察细胞生长及胶原纤维排列情况。 结果 MSCs在人耳脱细胞软骨支架上能立体培养成活 ,细胞分布不均 ,靠近培养液的一面细胞分布较多 ,其中大部分细胞进入已脱细胞的软骨陷窝内 ,每个陷窝内的细胞数为 1～ 2个。 结论 以鼠 MSCs为种子细胞 ,可在人耳脱细胞软骨支架上良好生长 ,构建组织工程软骨。
Objective To study the feasibility of using mice marrow stromal stem cells(MSCs) as seed cells for tissue engineering cartilage to embed the seed cells in acellular cartilage matrix of human auricle. Methods Acellular cartilage matrix was made from human auricle cartilage. The MSCs were isolated from the nucleated cells fraction of mice marrow by centrifuge.The MSCs were embedded in acellular cartilage matrix. After 10 day's combined culture, the specimens were observed with optical and electrical microscope.Results The MSCs could well proliferate in the acellular cartilage matrix. The cells were not well-distributed in acellular cartilage matrix. There were more cells in the peripheral part of the matrix than in the central part of the matrix. Most of the cells were in cartilaginous lacunae. There were 1 or 2 cells in every cartilaginous lacunae.Conclusion The MSCs can be used as seed cells of tissue engineering and can well proliferate in the acellular cartilage matrix and become tissue engineering cartilage.
出处
《中国修复重建外科杂志》
CAS
CSCD
2004年第3期214-216,共3页
Chinese Journal of Reparative and Reconstructive Surgery
基金
辽宁省教育厅高校科研基金资助项目 (2 0 1 2 2 1 76)~~
关键词
小鼠
骨髓基质干细胞
人耳脱细胞软骨
复合培养
脱细胞软骨支架
Tissue engineering cartilage Marrow stromal stem cells Acellular cartilage scaffold Complex culture
