摘要
目的 探讨脱细胞软骨生物支架材料（ACM）的制作方法，使其在孔隙率、比表面积、弹性模量等物理性质更加接近天然软骨。方法猪膝关节软骨冻干加工为粉末，0．25％胰酶消化，1％曲拉通洗脱，蒸馏水洗净冻干，8W312nm紫外线照射（UVI）后成型；扫描电镜观察孔径分布，压汞仪测定孔隙率等参数；INSTRON5544对ACM进行抗压弹性模量测定。结果脱细胞生物支架材料为白色，略微发黄，外表呈多孔疏松网状结构，脆性大并有一定弹性；ACM孔隙率为68．54％，平均孔径为47．13μm；ACM弹性模量为（1．05±0．38）MPa。结论ACM在孔隙率、比表面积、平均孔径等方面符合关节软骨支架材料的要求。
Objective To explore the more conformable cartilage biomaterial that conforms to the natural biomaterial in total porosity, total specific surface area, average pore radius, and elastic modulus. Methods The articular cartilage of pigs was processed into powder. The components of cells were eliminated by 0.25% Trypsin,1% TritonX-100 and distilled water. Finally the powder was frozen out by freezedryer and irradiated by 8 W 312 nm ultraviolet. The microcosmic images of ACM were examined by a scan electron microscope, and the parameters of pore radius distribution were measured by sorptomtic Instrument. The elastic modulus was measured by INSTRON 5544. Results The surface of the pale yellow ACM was porous and netty ,and the bouncy ACM was fragile. The average pore radius of ACM was 47.13μm,and the total porosity came to 68.54%. The elastic modulus of ACM came to （ 1.05± 0.38） MPa. Conclusion ACM accords with the biomaterial standards of cartilage tissue engineering in total porosity,total specific surface area, average pore radius, etc.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2009年第4期511-513,共3页
Chinese Journal of Experimental Surgery
基金
山西省国际技术合作项目（2007081037）
关键词
脱细胞
软骨材料
组织工程
Acellular
Cartilage material
Tissue engineering
