摘要
目的运用网络药理学的方法探讨黄芪治疗肾病综合征(NS)的分子作用机制。方法通过TCMSP数据库和文献挖掘获取黄芪主要活性成分,利用Pharm Mapper服务器、GeneCards和CoolGeN数据库预测和筛选黄芪活性成分的作用靶点。采用String数据库和Cytoscape软件绘制蛋白相互作用网络,通过Systems Dock Web Site对成分与靶点进行分子对接验证。借助DAVID数据库对靶点进行GO分析和KEGG通路分析,采用Cytoscape软件构建成分-靶点-通路-疾病网络。结果筛选得到黄芪25个活性成分,涉及47个作用靶点。网络分析结果表明,靶点主要涉及氧化应激、足细胞凋亡和脂质代谢等生物过程,通过调节Ras、Rap1、MAPK、PI3K-Akt和AGE-RAGE等信号通路来发挥治疗NS的作用。结论黄芪中黄酮类、皂苷类等活性成分可能是其治疗NS的物质基础,其作用机制涉及抗细胞凋亡、抗氧化应激和调节脂质代谢等。
Objective To explore the molecular mechanism of Astragali Radix in the treatment of nephrotic syndrome(NS)by the network pharmacology.Methods The main active ingredients of Astragali Radix were obtained through TCMSP and literature search.PharmMapper,Gene Cards and CoolGeN were used to predict and screen the targets of Astragali Radix.The protein interaction network was constructed using the String database and Cytoscape software.The relation between the main active ingredients and targets was validated by Systems Dock Web Site.The GO and KEGG pathways involved in the targets were analyzed by DAVID.The ingredients-targets-pathways-diseases network was constructed by Cytoscape software.Results The results showed that 25 active components and 47 targets of Astragali Radix were involved.The network results showed that the process of oxidative stress,podocyte apoptosis,lipid metabolism were mainly involved,which played a role in the treatment of nephrotic syndrome by adjusting the Ras,Rap1,MAPK,PI3 K-Akt,AGE-RAGE and other signal pathways.Conclusion This research method initially revealed that the active ingredients of flavonoids and saponins in Astragali Radix are the material basis for the treatment of NS.Its mechanism of action involved anti-oxidative stress,anti-apoptosis and regulation of lipid metabolism.
作者
杨柳
张王宁
刘月涛
李爱平
秦雪梅
YANG Liu;ZHANG Wang-ning;LIU Yue-tao;LI Ai-ping;QIN Xue-mei(Modern Research Center for Traditional Chinese Medicine,Shanxi University,Taiyuan 030006,China;College of Chemistry and Chemical Engineering,Shanxi University,Taiyuan 030006,China;Shanxi Key Laboratory of Active Constituents Research and Utilization of TCM,Taiyuan 030006,China)
出处
《中草药》
CAS
CSCD
北大核心
2019年第8期1828-1837,共10页
Chinese Traditional and Herbal Drugs
基金
国家自然科学基金资助项目:多效中药黄芪的定向药效化学成分研究(31570346)
山西省优秀人才科技创新项目:黄芪生长年限与化学成分积累规律研究(201605D211030)
山西省中药产业重点科技攻关项目:道地药材种质资源与规范化生产技术研究(201603D311101)
山西省药材行业标准-恒山黄芪药材与精品饮片商品等级标准研究(2014ZD0401)
关键词
网络药理学
黄芪
肾病综合征
分子机制
分子对接
network pharmacology
Astragali Radix
nephrotic syndrome
molecular mechanism
molecular docking
