摘要
为研究黄芪皂苷提取物对糖尿病大鼠肾脏的保护作用,将经链脲佐菌素诱导成功的32只糖尿病大鼠随机分为黄芪皂苷高、低剂量组、阳性对照组、模型组,分别以黄芪皂苷提取物200,50 mg·kg-1·d-1、缬沙坦25 mg·kg-1·d-1、生理盐水10 m L·kg-1·d-1灌胃,另取8只正常大鼠作为正常对照组(生理盐水10 m L·kg-1·d-1),连续给药6周。给药后每周称量大鼠体重,第2,4,6周检测空腹血糖;第6周代谢笼法检测24 h尿量及尿蛋白总量(U-TP);第6周末处死大鼠,心脏取血,检测血清中血尿素氮(BUN)、血肌酐(Scr)、尿酸(UA)、总胆固醇(CH)、甘油三酯(TG)等指标的含量;取双肾,计算肾肥大指数,观察肾脏病理切片。实验结果显示,模型组大鼠的血糖,24 h代谢值,肾肥大指数,U-TP,BUN,Scr,UA,TG较正常组均有显著性升高(P<0.01,P<0.05),病理改变明显加重;经黄芪皂苷提取物治疗后,高剂量组大鼠的24 h代谢值,肾肥大指数,U-TP,BUN,Scr,UA,TG均有明显下降(P<0.01,P<0.05),低剂量组大鼠的肾肥大指数,BUN,Scr,UA,TG亦下降明显(P<0.05),2组大鼠的肾脏病理改变均有所减轻。本实验结果说明黄芪皂苷提取物对糖尿病大鼠具有一定的肾脏保护作用。
To study the protective effect of astragalus saponin extracts （AS） on kidneys of diabetic rats. Totally 32 diabetic rats induced by streptozotocin （STZ） were divided into AS high and low dose groups, the positive control group and the model group （DM group） and orally administered with 50 mg · kg^-1 · d^-1 AS 200, 25 mg · kg^-1 · d^-1 valsartan, 10 mL · kg^-1 · d^-1 physiological saline, respectively. Another 8 healthy rats were collected in the normal control group （NC group, physiological saline 10 mL · kg^-1 · d^-1 ）. All rats were treated for consecutively 6 weeks. After the administration, the body weight was measured every week, the concen- tration of blood glucose was monitored on week 2, 4 and 6. The total urine and total urinary protein （U-TP） in 24 h were measured by the metabolic cage method on week 6; At the end of week 6, blood samples were collected from hearts to detect blood urea nitrogen （BUN）, serum creatinine （Scr）, uric acid （UA）, total cholesterol （CH） triglyceride （TG） by biochemical methods. Kidneys were collect to calculate the kidney hypertrophy index and observe the pathological sections. The laboratory results show that in the DM group, the blood glucose, metabolic cost in 24 h, kidney hypertrophy index, U-TP, BUN, Scr, UA, TG were significantly higher than that in the NC group （P 〈0.01, P 〈0.05）, with significant pathological changes; After the intervention with AS, the metabolic value in 24 h, kidney hypertrophy index, U-TP, BUN, Scr, UA, TG were significantly lower in the high dose group （P 〈 0.01, P 〈 0.05） , and the kidney hypertrophy index, BUN, Scr, UA, TG in the low dose group were also significantly lower （P 〈 0.05）, with slight reduction in renal pathological changes in both groups. In conclusion, Astragalus saponin extracts have a certain protective effect on kidneys of diabetic rats.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2015年第10期2014-2018,共5页
China Journal of Chinese Materia Medica
基金
浙江省中医药科技计划项目(2013ZB033)
浙江省大学生科技创新活动计划暨新苗人才计划项目(2014R410053)
浙江省教育厅研究项目(Y201226284)
关键词
黄芪皂苷
糖尿病
肾脏
保护作用
astragalus saponin
diabetes
kidney
protective effect
