摘要
目的:探讨槲皮素(quercetin,QU)联合顺铂(DDP)对胃癌SGC-7901细胞增生、凋亡及凋亡相关基因Bcl-XL mRNA表达的影响. 方法:用槲皮素和顺铂处理胃癌SGC-7901细胞后,应用光镜、电镜、MTT法、流式细胞仪和RT-PCR 技术研究胃癌细胞的形态学变化、生长抑制、诱导凋亡和对凋亡相关基因Bcl-XL mRNA表达的影响. 结果:药物作用于细胞24 h后,可看到较为典型的细胞凋亡形态学变化.DDP和QU均可以抑制胃癌SGC-7901 细胞增生或诱导其凋亡,且有时间和剂量依赖效应,联合应用具有协同效应.联合组的诱导凋亡率及增生抑制效应显著高于单用DDP组(P<0.01),干预48 h后,Bcl- XLmRNA的表达下调,QU可以加强下调作用. 结论:人胃癌SGC-7901细胞体外实验中,QU和顺铂可以抑制其增生并诱导凋亡,二者联合应用有一定的协同效应,且呈剂量依赖关系.
AIM: To investigate the effects of quercetin combined with cisplatin on the proliferation and apoptosis of, and the expression of apoptosis-related gene Bcl-XL in human gastric cancer SGC-7901 cells. METHODS: After treated with quercetin (QU), cisplatin (DDP) or both (QU-DDP), the viability of SGC-7901 cells was evaluated by MTT assay. Cell cycle distribution and apoptosis were detected by flow cytometry. Cell morphology was examined under light microscope and electron microscope. The expression of Bcl-XL mRNA was detected by the reverse transcription polymerase chain reaction (RT-PCR). RESULTS: After exposure to different drugs for 24 h, SGC-7901 cells manifested typical morphological features of apoptosis. Both DDP and QU inhibited the proliferation and induced the apoptosis of SGC7901 cells in a time and concentration-dependent manner. QU combined with DDP synergistically enhanced cell death. The apoptosis was more pronounced in cells treated with both drugs (QU: 1μmol/L, DDP: 80 μmol/L) than DDP alone (80μmol/L) (19.93±0.07 vs5.16±0.11, P<0.01). After exposure to DDP for 48 h, Bcl-XL mRNA expression was down-regulated, which was promoted by QU. CONCLUSION: QU combined with DDP effectively inhibits proliferation and induces apoptosis of the human gastric cancer SGC-7901 cells in vitro in a dose-dependent manner.
出处
《世界华人消化杂志》
CAS
北大核心
2005年第3期303-307,共5页
World Chinese Journal of Digestology
