摘要
为了快速、准确识别不同甘蓝自交系的遗传背景,提高回交育种效率,利用36对多态性EST-SSR引物对8个甘蓝变种的101份自交系进行扩增,应用NTSYSpc2.11a进行遗传背景分析表明,不同甘蓝变种种内平均遗传相似系数都大于0.55,变种间遗传相似系数为0.45~0.59,101份自交系在遗传相似系数0.62处基本可聚为8个类群。在此基础上,选出23份具有代表性的自交系材料,增加筛选获得多态性稳定的50对EST-SSR引物,初步建立了一套包含86对引物的甘蓝背景选择标记,并应用于甘蓝自交亲和性的回交选择。
For the purpose of the rapid and accurate identification of genetic background of cabbage inbred lines and the improvement of backcross breeding efficiency,we performed PCR amplification of 36 primer pairs in 101 inbred lines from eight varieties in Brassica oleracea and analyzed their genetic background using software NTSYSpc2.11 a. Results showed that all the average coefficients of intravariety for the eight varieties are bigger than 0.55,and that of inter-variety vary from 0.45 to 0.59. The 101 B. oleracea accessions can be divided into eight groups with the coefficient of 0.62. Based on these results,23 out of 101 inbred lines were selected and 50 EST-SSR markers were added. At last,a set of 86 markers for genetic background selection was preliminarily obtained and used successfully for the backcross selection of cabbage self-compatibility.
出处
《园艺学报》
CAS
CSCD
北大核心
2014年第8期1620-1630,共11页
Acta Horticulturae Sinica
基金
国家重点基础研究发展计划(‘973’)项目(2012CB1139)
国家‘863’计划课题(2012AA100101
2012AA100202)
国家现代农业产业技术体系建设专项资金项目(CARS-25)
中央级公益性科研院所基本科研业务费专项资金项目(1610032011011
014JB02-004)
农业部园艺作物生物学与种质创制重点实验室项目
