摘要
通过与opaque-2(o2)基因紧密连锁的SSR分子标记phi057对X178和CA335回交群体回交一代BC1F1和回交二代BC2F1o2基因的前景选择,发现可以在苗期对回交群体进行o2基因的检测和追踪,可提高选择的准确性。通过利用AFLP分子标记进行轮回亲本的背景选择,表明利用AFLP分子标记辅助选择可以使回交二代与轮回亲本的相似性比回交一代与轮回亲本的相似性显著增加,使位点进一步纯合,选择出与轮回亲本具有较高相似性单株个体,从而加快优质蛋白玉米育种进程,为分子标记辅助选择QPM提供了重要理论依据。
The SSR markers phi057 linked tightly with opaque-2 （o2） can be used effectively in the quality protein maize （QPM） development. In the study, the SSR primer phi057 was used in the evaluation of foreground and background of BC1F1 and BC2 F1 which were crossed by （CA335 × X178）. It was proved that SSR is a powerful tool which dects o2 gene of QPM. The AFLP primers were used to detect background in BC1 F1 and BC2F1 of the crosses. It was proved that the similarity of BC2F1 increases more apparently than similarity of BC1 F1, and more heterozygous loci tend to homozygous. Molecular Markers Assisted Selection （MAS） can expedite breeding program. It offers important theoretical method for Molecular Markers Assisted Selection of QPM.
出处
《新疆农业大学学报》
CAS
2005年第3期5-8,共4页
Journal of Xinjiang Agricultural University
基金
农业部引进国际先进农业科学技术项目(2003-Q03)
关键词
优质蛋白玉米(QPM)
分子标记辅助选择
SSR
AFLP
quality protein maize （QPM）
marker assisted selection
simple sequence repeat （SSR）
amplified fragment length polymorphism （AFLP）
