摘要
目的:建立测定血府逐瘀口服液中苦杏仁苷的含量的方法。方法:采用高效液相色谱-蒸发光散射检测器法。色谱柱为十八烷基硅烷键合硅胶为填充剂,流动相为甲醇-水(25:75),流速控制在1ml/分,柱温控制在30℃;蒸发光散射检测器的漂移管温度控制105℃,空气或氮气作为其中的雾化气体,其流速控制在3.0L/分。结果:苦杏仁苷的进样量0.856~4.28μg,其峰面积的自然对数与进样量的自然对数具有良好的线性关系(r=0.9995),平均回收率99.06%,RSD=1.39(n=6)。结论:该方法灵敏、准确,可用于血府逐瘀口服液中苦杏仁苷的含量测定。
Objective:To develop a method for the content determination of amygdalin in Xue Fu Zhu Yu oral liquid.Methods:High performance liquid chromatography-evaporative light-scattering detector method was used.The chromatographic column was octadecylsilane chemically bonded silica as the filler,the moving phase was methanol-water(25:75),the flow velocity was 1ml one minute,the column temperature was 30℃.The drift tube temperature of evaporative light-scattering detector was 105℃,air or nitrogen was as the atomizing gas,its flow velocity was 3.0L one minute.Results:The incoming sample amount of amygdalin was0.856~4.28μg,the natural logarithm of peak area and the natural logarithm of incoming sample amount had good linear relationship(r=0.9995),the average recovery was 99.06%,RSD=1.39(n=6).Conclusion:This method is sensitive,accurate,can be used for the content determination of amygdalin in Xue Fu Zhu Yu oral liquid.
关键词
血府逐瘀口服液
苦杏仁苷
高效液相色谱法
蒸发光散射检测器
含量测定
Xue Fu Zhu Yu oral liquid
Amygdalin
High performance liquid chromatography
Evaporative light-scattering detector
Content determination
