摘要
目的:分析铜死亡基因在多发性硬化(MS)发生发展过程中与免疫浸润的相关性,并进行中药预测,为铜死亡在MS免疫浸润方面的机制研究及中药干预免疫调节提供理论依据。方法:通过GEO数据库下载MS的芯片并进行标准化处理;基于处理后的数据进行免疫细胞和功能的提取与量化,分析免疫细胞和功能的相关性及差异性;同时筛选出与MS相关的铜死亡基因,构建风险模型,并对其进行富集分析;对铜死亡基因及免疫相关的生物过程进行中药预测。结果:①免疫细胞间,滤泡辅助性T细胞和B细胞呈最强正相关;免疫功能间,低度炎症和Ⅰ型IFN反应呈最强正相关;②B细胞、辅助性T细胞、人类白细胞抗原在MS患者中丰度显著降低,而主要组织相容性复合体Ⅰ类分子、低度炎症、Ⅰ型IFN反应丰度显著升高;③与MS相关的铜死亡基因为SLC31A1、PDHA1、NLRP3、MTF1、GLS和DBT,其中DBT最有可能是MS的风险因子;④MS发生发展涉及IL-4的产生、辅助性T细胞分化、急性炎症反应等生物过程,与精氨酸生物合成、柠檬酸循环、丙酸代谢等通路相关;⑤半夏、丹参、姜黄等中药可能作为其潜在的分子药物来源。结论:铜死亡基因的表达与MS相关免疫细胞和功能密切相关,可为MS的基础研究提供支持。
Objective:To analyze the correlation between cuproptosis genes and immune infiltration during the occurrence and development of multiple sclerosis(MS),and to predict the traditional Chinese medicine,to provide theoretical basis for the mechanism study of cuproptosis in MS immune infiltration and Chinese medicine to intervene in immune regulation.Methods:The gout chip of MS was downloaded from the GEO database and standardized;based on the processed data,immune cells and functions were extracted and quantified,and the correlation and differences of immune cells and functions were analyzed;at the same time,cuproptosis genes related to MS were screened out,a risk model was constructed,and enrichment analysis was carried out;the prediction of cuproptosis genes and immune-related biological processes were carried out.Results:①Among immune cells,T follicular helper cell and B cell showed the strongest positive correlation;among the immune functions,parainflammation and typeⅠIFN reponse showed the strongest positive correlation;②B cell,T helper cell and human leukocyte antigen were lowly expressed in MS patients,while major histocompatibility complex classⅠ,parainflammation and typeⅠIFN response were significantly expressed;③The cuproptosis genes associated with MS were SLC31A1,PDHA1,NLRP3,MTF1,GLS and DBT,of which DBT was the most likely risk factor for MS;④The occurrence and development of MS involves biological processes such as IL-4 production,T-helper cell differentiation,and acute inflammatory response,which were related to pathways such as arginine biosynthesis,citrate cycle,and propanoate metabolism;⑤Banxia,Danshen,Jianghuang and other traditional Chinese medicines may be used as potential molecular drug sources.Con⁃clusion:The expression of cuproptosis gene is closely related to MS-related immune cells and functions,which can provide support for the basic research of MS.
作者
蒙毅
谭文澜
江颖
吴月霞
傅有学
曹康
张琼心
陈晓丽
MENG Yi;TAN Wenlan;JIANG Ying;WU Yuexia;FU Youxue;CAO Kang;ZHANG Qiongxin;CHEN Xiaoli(Ruikang Hospital Affiliated to Guangxi University of Traditional Chinese Medicine,Nanning 530011,China;Guangxi University of Traditional Chinese Medicine,Nanning 530001,China)
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2024年第8期1718-1725,I0002,共9页
Chinese Journal of Immunology
关键词
铜死亡
多发性硬化
免疫浸润
生物信息学
免疫功能
Cuproptosis
Multiple sclerosis
Immune infiltration
Bioinformatics
Immune function
