摘要
目的 探讨麦冬皂苷对大鼠肝部分切除后肝再生的影响及对Wnt/β-catenin信号通路的调节作用。方法 42只SD大鼠随机分为假手术组、模型组和麦冬皂苷组各14只。术前5 d麦冬皂苷组尾静脉注射麦冬皂苷20 mg/kg,假手术组和模型组尾静脉注射等量生理盐水,1次/d,连续5 d。末次给药后第2天,模型组和麦冬皂苷组切除肝脏左叶和中叶;假手术组仅打开腹腔,暴露肝脏,不切除肝组织。术后1、4 d每组取7只大鼠,称体质量和肝脏质量,计算肝脏指数;检测血清谷丙转氨酶(GPT)、谷草转移酶(GOT)水平,采用ELISA法检测血清白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)水平,采用Western blot法检测肝组织增殖细胞核抗原(PCNA)、Ki-67、Wnt2及细胞质和细胞核β-catenin蛋白相对表达量。结果 术后1、4 d,麦冬皂苷组[(1.28±0.13)%、(2.01±0.14)%]、模型组[(1.22±0.11)%、(1.68±0.14)%]肝脏指数均低于假手术组[(2.36±0.15)%、(2.37±0.12)%](P<0.05),麦冬皂苷组高于模型组(P<0.05);术后1、4 d,麦冬皂苷组、模型组血清GPT、GOT、IL-1β、TNF-α水平均高于假手术组(P<0.05),麦冬皂苷组均低于模型组(P<0.05);麦冬皂苷组、模型组术后4 d肝脏指数均高于术后1 d(P<0.05),血清GPT、GOT、IL-1β、TNF-α水平均低于术后1 d(P<0.05),假手术组术后4 d与术后1 d比较差异均无统计学意义(P>0.05)。术后1、4 d,麦冬皂苷组、模型组肝组织PCNA、Ki-67、Wnt2、细胞核β-catenin蛋白相对表达量均高于假手术组(P<0.05),细胞质β-catenin蛋白相对表达量均低于假手术组(P<0.05);麦冬皂苷组肝组织PCNA、Ki-67、Wnt2、细胞核β-catenin蛋白相对表达量均高于模型组(P<0.05),细胞质β-catenin蛋白相对表达量低于模型组(P<0.05)。麦冬皂苷组、模型组术后4 d肝组织PCNA、Ki-67、Wnt2、细胞核β-catenin蛋白相对表达量均高于术后1 d(P<0.05),细胞质β-catenin蛋白相对表达量均低于术后1 d(P<0.05);假手术组
Objective To investigate the effect of ophiopogonin on liver regeneration after partial hepatectomy in rats and the role in regulating Wnt/β-catenin signaling pathway.Methods Forty-two SD rats were randomly divided into sham-operation group,model group and ophiopogonin group,with 14 rats in each group.Ophiopogonin group was injected with 20 mg/kg via tail vein 5 d before operation,while sham-operation group and model group were injected with the same amount of normal saline once a day for 5 consecutive days.On the next day after the last administration,the left lobe and middle lobe of liver were resected in model group and ophiopogonin group.The rats in sham-operation group were only opened the abdominal cavity,and exposed the liver,without resecting the liver.On the 1st and 4th days after operation,7 rats in each group were measured the body mass and liver mass,and the liver index was calculated.The serum glutamic-pyruvic transaminase(GPT)and glutamic-oxaloacetic transaminase(GOT)levels were detected.The serum interleukin-1β(IL-1β)and tumor necrosis factor-α(TNF-α)were detected by ELISA.The relative expressions of proliferating cell nuclear antigen(PCNA),Ki-67,Wnt2,and cytoplasmic and nuclearβ-catenin proteins were detected by Western blot.Results The liver indexes were lower in ophiopogonin group[(1.28±0.13)%,(2.01±0.14)%]and model group[(1.22±0.11)%,(1.68±0.14)%]than those in sham-operation group[(2.36±0.15)%,(2.37±0.12)%](P<0.05),and were higher in ophiopogonin group than those in model group on the 1st and 4th days after operation(P<0.05);were higher on the 4th day after operation than those on the 1st day after operation in ophiopogonin group and model group(P<0.05),and showed no significant differences in sham-operation group on the 4th day after operation compared with those on the 1st day after operation(P>0.05).The levels of serum GPT,GOT,IL-1βand TNF-αwere higher in ophiopogonin group and model group than those in sham-operation group(P<0.05),and lower in ophiopogonin group than those in
作者
张鹏
陈昆
宋登辉
李东亮
杨栋强
ZHANG Peng;CHEN Kun;SONG Denghui;LI Dongliang;YANG Dongqiang(Department of Hepatobiliary Pancreatic and Splenic Surgery,Zhumadian Central Hospital,Zhumadian,Henan 463000,China;Departmentof Infectious Diseases,Henan Provincial People's Hospital,Zhengzhou University People's Hospital,Zhengzhou,Henan 450003,China)
出处
《中华实用诊断与治疗杂志》
2024年第6期552-556,共5页
Journal of Chinese Practical Diagnosis and Therapy
基金
河南省医学科技攻关计划联合共建项目(LHGJ20210019)。
