摘要
目的利用细粒棘球绦虫(Echinococcus granulosus,E.granulosus)感染小鼠模型,结合70%肝切除手术,探索E.granulosus感染对肝脏组织再生的影响,初步探讨MyD88/IL-23在E.granulosus感染小鼠术后肝再生中的表达及意义。方法建立了E.granulosus皮下感染后的小鼠70%肝切除模型。选择18只E.granulosus感染的小鼠,以及18只作为对照的健康小鼠。在手术后的不同时间点,比较2组小鼠的肝脏再生量和再生率。ELISA法监测不同时间点小鼠血清中的MyD88和IL-23水平及肝功能指标AST和ALT的变化。结果与对照组小鼠比较,E.granulosus感染小鼠术后肝再生量和再生率降低,在术后的12 h、1 d和2 d表现出显著性差异。与对照组小鼠比较,E.granulosus感染小鼠外周血中AST和ALT水平在术后1 d明显升高;MyD88和IL-23水平在术后2 h明显升高。结论E.granulosus感染显著减缓了小鼠肝脏切除术后的再生过程,与此同时,感染小鼠的MyD88和IL-23水平显著增加,暗示它们可能在此过程中起作用。
Objective To explore the influence of Echinococcus granulosus,(E.granulosus)infection on liver regeneration in hepatectomy model in mice,and to investigate the expression of MyD88/IL-23 in the liver regeneration of E.granulosus-infected mice post hepatectomy and its significance.Methods A mouse model with 70%hepatectomy following subcutaneous infection of E-.granulosus was established.A total of 18 E.granulosus-infected mice and 18 healthy mice as controls were chosen for the surgery.The amount and rate of liver regeneration at different time points post-surgery in mice between the two groups were analyzed.The expression levels of MyD88 and IL-23 in mouse serum at different time points were measured by ELISA.The changes in hepatic function indicators AST and ALT were also monitored.Results Compared to the control mice,the E-.granulosus-infected mice demonstrated decreased liver regeneration amount and regeneration rate post hepatectomy,with significant differences apparent at the time points of 12 hours,1 day,and 2 days post-surgery.In comparison to the control mice,the E.granulosus-infected mice exhibited markedly increased levels of AST and ALT in peripheral blood 1 day post-surgery,and notably increased levels of MyD88 and IL-232 hours post-surgery.Conclusions E.granulosus infection significantly decelerates the liver regeneration process following hepatectomy in mice,which may through increasing the levels of MyD88 and IL-23.
作者
陈骏
于鹏
CHEN Jun;YU Peng(First Department of Surgery,Fourth Clinical Medical College,Xinjiang Medical University,Urumqi,830000,China)
出处
《医学分子生物学杂志》
CAS
2024年第4期361-365,共5页
Journal of Medical Molecular Biology
基金
新疆维吾尔自治区自然科学基金(No.2022D01C316)。
关键词
细粒棘球绦虫
髓样分化因子88
白介素-23
肝再生
Echinococcus granulosus
myeloid differentiation factor 88
interleukin-23
liver regeneration
