摘要
目的 建立和评价腹部术后疲劳综合征大鼠模型。方法 采用手术切除大鼠部分肝脏 ,术后观察动物的一般情况、鼠尾悬挂实验、负重游泳力竭实验 ,血清蛋白、转铁蛋白、血清铁 ,小肠黏膜损伤病理评价 ,肝脏白蛋白基因表达等指标。结果 70 %肝切除实验大鼠一般情况差、体力耐受力下降 ,有一定程度的抑郁表现 ,并在营养学指标、小肠黏膜应激反应损伤和肝清蛋白基因表达等方面发生明显改变。结论 切除 70 %肝脏制作大鼠动物模型 ,具有临床术后疲劳综合征的基本特征 ,可作为研究腹部手术后疲劳综合征实验动物模型。
Objective To establish and assess a model of abdominal postoperative fatigue syndrome (POFS) in rats. Methods After 70% hepatectomy was performed, the following observations of the animals were made:general condition, rat tail suspension test,weight carrying swim fatigue test,serum levels of albumin,ferrition,and iron,pathologic assessment of injury of small intestinal mucosa and hepatic albumin gene expression .Results After 70% hepatectomy of the rats,their general candition was poor,the level of physical tolerance decreased,they showed a certain amount of depression,and marked changes were found in nutritional index,stress injury of small intestinal mucosa and hepatic albumin gene expression.Conclusions A 70% hepatectomy rat model has the basic characteristics of clinical abdominal POFS, and can be used as an experimental animal model for the study of abdominal POFS.
出处
《中国普通外科杂志》
CAS
CSCD
2005年第1期40-43,共4页
China Journal of General Surgery
基金
广东省中医药管理局科研资助项目 (0 9951 1 )。
关键词
腹部/外科学
疲劳综合征
肝切除术
疾病模型
动物
ABDOMEN/surg
POSTOPERATIVE FATIGUE SYNDROME
HEPATECTOMY
DISEASE MODELS,ANIMAL
