摘要
目的以SD大鼠和C57BL/6J小鼠为实验对象,建立术后疲劳综合征(POFS)模型。方法手术切除SD大鼠或C57BL/6J小鼠70%的肝,制备POFS动物模型。于术后不同时间观察动物的一般状态,采用自发活动、抓力、跑台运动和负重游泳实验以及生理信号遥测技术对模型动物的活动性、肌肉力量、运动耐力和睡眠结构进行综合评价,以确定敏感种属和检测指标;在小鼠POFS模型中,评价经典的抗疲劳药物咖啡因(3 mg·kg^(-1),ip)对小鼠活动性和跑台行为的影响,并采用试剂盒检测小鼠血糖、肌糖原和肝糖原含量的变化。结果大鼠POFS模型中,与正常对照组相比,模型组大鼠术后当天眯眼评分显著增加(P<0.01),术后第1,3,5和7天体重显著降低(P<0.05,P<0.01),术后第1天活动性显著降低(P<0.01),术后第1~3天抓力及术后第3和4天负重游泳力竭时间无显著差异;睡眠分析结果表明,POFS大鼠术后睡眠紊乱,表现为手术当天及术后第1天大鼠活动清醒时间百分比显著减少(P<0.01,P<0.05)、慢波睡眠时间百分比显著增加(P<0.01,P<0.05)。小鼠POFS模型中,与正常对照组相比,模型组小鼠手术当天和术后第1天眯眼评分显著增加(P<0.01,P<0.05),术后第1,3,5和7天体重显著降低(P<0.05,P<0.01),术后第1和3天活动性显著下降(P<0.01),术后第1,3和5天抓力显著降低(P<0.01),术后第3和5天跑台运动距离显著减少(P<0.01),术后第4,6和8天负重游泳力竭时间无显著差异。与小鼠POFS模型组相比,模型+咖啡因组小鼠术后第1和3天活动性(P<0.01,P<0.05)和术后第3天跑台运动距离显著增加(P<0.01),肝糖原和肌糖原含量显著增加(P<0.05)。结论采用70%肝切除术可成功构建大鼠和小鼠POFS模型,模型稳定可靠,具有良好的可重复性。小鼠POFS模型维持时间长,且可采用活动性、运动耐力和肌肉力量为客观指标进行综合评价,且对经典的抗疲劳药物咖啡因敏感,可作为POFS病理生理机制�
OBJECTIVE To establish postoperative fatigue syndrome(POFS)models in SD rats and C57BL/6J mice.METHODS POFS models in rats and mice were developed by 70%hepatectomy.The fatigue state,muscle strength,exercise endurance and sleep structure of the model animals were evaluated by activity test,grip test,treadmill test,weight-loaded swimming test and DSI physiological signal telemetry technology at different time points after operation so as to determine the sensitive species and detection indexes.In the mouse POFS model,the effects of the classical anti-fatigue drug caffeine(3 mg·kg^(-1),ip)on the activity and treadmill behavior of mice were evaluated,and the changes of blood glucose,muscle glycogen and liver glycogen in mice were detected by the kit.RESULTS In the POFS model of rats,compared with the normal control group,the squint score increased significantly on the day of operation(P<0.01),the body mass decreased significantly on the 1^(st),3^(rd),5^(th)and 7^(th)day postoperatively(P<0.05,P<0.01),and the activity decreased significantly on the first postoperative day(P<0.05).There was no significant difference in grip strength on the 1^(st)and 3^(rd)day or in exhaustive swimming time on the 3^(rd)and 4^(th)day following operation.The results of sleep analysis showed that postoperative sleep disorder occurred in POFS rats,as was demonstrated by the significantly lower percentage of active awake time(P<0.01,P<0.05)and the significantly higher percentage of slow wave sleep time on the day of operation and the first day following operation(P<0.01,P<0.05).Compared with the normal control group,the squint score in the POFS model in mice increased significantly on the day of operation and the first day following operation(P<0.01,P<0.05),the body mass decreased significantly on the 1^(st),3^(rd),5^(th)and 7^(th)day following operation(P<0.05,P<0.01),so did the activity on the 1^(st)and 3^(rd)day following operation(P<0.01),the grip strength on the 1^(st),3^(rd)and 5^(th)day following operation(P<0.01),and the treadmill
作者
张雯雯
尹雪蕾
王洛萱
李劲草
李硕
张杨
李迎
张有志
王恒林
薛瑞
ZHANG Wen-wen;YIN Xue-lei;WANG Luo-xuan;LI Jin-cao;LI Shuo;ZHANG Yang;LI Ying;ZHANG You-zhi;WANG Heng-lin;XUE Rui(Graduate School of Hebei North College,Zhangjiakou 075000,China;The 8th Medical Center of Chinese PLA General Hospital,Beijing 100094,China;State Key Laboratory of Toxicology and Medical Countermeasures,Institute of Pharmacology and Toxicology,Academy of Military Medical Sciences,Beijing 100850,China)
出处
《中国药理学与毒理学杂志》
CAS
北大核心
2021年第2期123-131,共9页
Chinese Journal of Pharmacology and Toxicology
基金
国家科技重大专项(2018ZX09739-008)。
关键词
术后疲劳综合征
动物模型
肝切除术
postoperative fatigue syndrome
animal model
liver resection
